Microfilariae Flashcards Preview

Filariae

› Wuchereria bancrofti

› Brugia malayi

› Loa loa

› Onchocerca volvulus

› Mansonella spp.

Microfilariae

When a parasite appears during certain times of the day. 

›more prevalent in peripheral blood at specific times of day or evening

›times appear to coincide with the usual feeding patterns of intermediate host

›nocturnal

› nighttime

›diurnal

› daytime

› mix 1 mL of whole blood w/ 10 ml  of 2% formalin

› spin 1 min @ 500g

› make thick smears from sediment

›distribution of nuclei within tip

›presence/absence of a sheath

›Granulomatous lesions

›Eosinophilia

›Fever/chills

›Elephantiasis

› obstruction of lymphatic circulation

› enlargement of legs, breasts, scrotum

›Calabar swellings

› swelling of subcutaneous tissue

Wuchereria bancrofti microfilariae

Brugia malayi

› Plasmodium

› Babesia

› Cryptosporidium

› Pneumocystis

› Toxoplasma

› Isospora

› Sarcocystis

› P. falciparum (most dangerous)

› P. vivax

› P. ovale

› P. malariae

merozoites

gametocytes

›Giemsa-stained peripheral blood smears examined under oil immersion

› thick smears (screening)

› thin smears (differentiation)

›Timing of blood collection is critical

› greatest # in-between paroxyms

Plasmodium falciparum

Plasmodium falciparum

P. falciparum ring-form trophozoites

P. falciparum gametocyte

Plasmodium falciparum

Plasmodium vivax

Plasmodium vivax

P. vivax trophozoite

P. vivax immature schizont

P. vivax macrogametocyte

P. vivax microgametocyte