Microbiology Test 1 Flashcards Preview

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Flashcards in Microbiology Test 1 Deck (31)
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1

This mycobacteria does not grow in vitro

M. leprae

2

pigmented only after exposure to light (Runyoun Grp)

Group1 - Photochromogen

3

pigmented in dark and light (Runyoun Grp)

Group II - scotochromogen

4

non-pigmented (Runyoun Grp)

Group III - nonphotochromogen

5

Runyoun Group 4 characteristic

Rapid Growers

6

Etiological agent of TB

Mycobacterium Tuberculosis

7

Many TV organisms are becoming Resistant to antibiotics mostly due to?

Lack of patience compliance. DIRECT OBSERVATION THERAPY(monitor patient ingestion using antibiotics) preferred

8

[M. fortuitum-chelonae Complex]These three clinically significant species have what key characteristic in common?
M. fortuitum
M. chelonae
M. abscessus

RAPID GROWERS

9

Etiologic agent of leprosy

Hansen disease

10

(T/F) M.Tuberculosis is Niacin positive

True, M.Tuberculosis is Niacin positive

11

What antibiotics are prescribed to treat TB?

9 months with isoniazid & rifampin
once/day for 1st month then 2/per week

if R to isoniazid or rifampin then use
streptomycin, ethambutol

12

Organisms in the Mycobacterium Avium Complex include?

M. avium
M. intracellulare
M. kansasii

13

Primarily disease in poultry, swine
Found in natural waters, house dust
Large increase due to AIDS
pulmonary or disseminated
Both species very similar
not differentiated

M. avium- intracellulare

14

Common cause of non-TB mycobacterial pulmonary infection
isolated from water
human source unknown
using multidrug regimen to treat
rifampin
isoniazid
ethambutol

M. kansasii

15

Disease of fish
Cutaneous infections in humans
tender red or red-blue nodules
contact with poorly chlorinated & salt H2O
“swimming pool granuloma”

M. marinum

16

M. fortuitum-chelonae Complex (RAPID GROWERS) includes these organisms.

M. fortuitum
M. chelonae
M. abscessus***

17

“Tap water bacillus”
water taps, soil
rare cause of disease
frequently isolated as transient resident

M. gordonae

18

affects skin, mucus membranes and nerves
rare in US, western Europe
big problem elsewhere
transmission requires prolonged contact with infected person

Hansens Disease (leprosy)

19

What are the two forms of leprosy?

Tuberculoid and Lepromatous. Lepromatous being the much more malignant of the two.

20

Newer Mycobacteria Identification Methods

Radiometric culture system (BACTEC)
Nucleic acid probes (rRNA)
PCR
Chromatography

21

Tuberculin skin test at least

annually

22

(T/F) not all disinfectants are tuberculocidal

True.

phenol-soap
sodium hypochlorite (bleach)
formaldehyde
5% phenol

is used. Also disposable equipment.

23

(T/F) You can refrigerate specimens for up to 24 hours

True. You can refrigerate specimens for up to 24 hours

24

[Specimens] How many positive smears is diagnostic for Mycobacteria?

2 or more smears out of 3 is diagnostic

none or 1 smear positive then
need more specimens

25

What is the preferred urine sample for Mycobacteria?

first AM midstream preferred (can refrigerate)

26

Why are mycobacteria so hard to stain?

** high lipid content in cell wall protective

27

A nonpigmented mycobacteria is isolated that reduces nitrate to nitrite and is niacin-positive. What should you suspect?

M. Tuberculosis

28

This detects a cell-mediated immune response to mycobacterial antigens

Skin test for TB

29

Group 1 Photochromogen species include

M. Kansasii, M. Marinum, and M simiae

30

Group 3 nonchromogen species include

M. Avium, M. intracellularae, and M. ulcerans