The SBB stain is the most sensitive stain for what precursor cells?
Granulocytic precursors
The SBB staining pattern parallels what other stain?
Myeloperoxidase
TdT, terminal deoxynucleotidyl transferase, is found in 90% of what type of leukemia?
Acute lymphoblastic leukemia
What does SBB stain?
Phospholipids, neutral fats, and sterols
The _______ stain is more specific than the _______ stain.
The Myeloperoxidase stain is more specific than the Sudan black B stain
The ________ stain is more sensitive than the _______ stain.
The Sudan black B stain is more sensitive than the Myeloperoxidase stain.
What chromosome abnormality is found in Precursor B-cell ALL?
t(1;19)
A positive nonspecific esterase stain indicates what type of differentiation?
Monocytic
A strong PAS reaction supports the diagnosis of ________
Erythroleukemia
The acid phosphatase/tartrate resistant stain is used for making diagnosis of?
Harry Cell Leukemia
Cytoplasmic marker studies are useful in assessing cell lineage in?
Acute Lymphoblastic Leukemia
What is the classification of anemia in CLL?
Normocytic/normochromic
In leukemia, the presence of predominantly mature malignant cells is classified as _________
Chronic
Diagnosis of CLL can be confirmed immunologically by the use of monoclonal antibodies that detect _____________ antigens
Cluster differentiation (CD)
Most patients are diagnosis in which phase of CML?
Chronic
Acute erythroleukemia is characterized by an abnormal proliferation of what precursors?
erythroid and myeloid
In CML, transformation to a more critical phase is evident by the appearance of which cells?
Blasts
The morphology of acute lymphoblastic leukemia is evaluated on what type of smear?
Bone marrow aspirate
On Romanowsky-stained smears, how do Auer rods appear?
Pink or purple staining rods
Cytochemical stains were performed on bone marrow smears from an acute leukemia patient. All blasts were TdT negative. The majority of the blasts showed varying amounts of SBB positivity. 50% of them stained positive for NSE. What type of leukemia is indicated?
Acute myelomonocytic leukemia
True or False:
Age of the patient, nuclear chromtin pattern, and presence of nucleoli can all be used to differentiate CLL from ALL.
True
True or False:
Chronic leukemias are found primarily in children
False
What is the chromosomal abnormality in CML?
t(9;22)
What is the most common chromosomal abnormality in B-CLL?
Trisomy 12
What are required hematologic findings for a diagnosis of CLL?
- 10,000/uL lymphocytes in the peripheral blood
- 30% lymphocytosis in the bone marrow
What type of leukemia is associated with these cells?
B-cell Acute Lymphocytic Leukemia L3
Identify the leukemia
Chronic lymphocytic leukemia
What is the only curative treatment for CML?
Hematopoetic stem cell transplant
The prominent laboratory feature representative of CML is?
Leukocytosis with a left shift
Which FAB classification of ALL represents a heterogeneous population and is morphologically similar to AML M1?
ALL L2
