Flashcards in Lec 9. Chromosome variation II Deck (87)
What is metacentric?
centromere in the middle
What is submetacentric?
chromosome with somewhat smaller arm
What is acrocentric?
chromosome with small arm
What is telocentric?
chromosome with no arm
Which chromosomes have a satellite that can be recognized by a secondary constriction?
What does P stand for pertaining to chromosomes?
P is the short arm
What does q stand for pertaining to chromosomes?
q is the long arm
What does the satellite contain?
NOR (nucleolar organizer region)
What does NOR do?
Can be detected by silver staining and is a useful landmark for chromosome indentification
What do chromosome mutations include?
Rearrangements, aneuploids and polyploids
What are the three steps to karyotyping?
Chromosomes prepared from actively dividing cells. Halted in metaphase with colchicine. Chromosomes arranged according to size.
What are the 4 types of banding?
What stain is used for G bands?
What stain is used for Q bands?
What do C bands show?
Reveals centromeric heterochromatin
What do R bands show?
Region rich in C-G base pairs
What does G banding show?
Heterochromatin stains intensely(A-T rich) and euchromatin (G-C) stains lightly.
What does Q banding show?
Florescent stain for A-T rich regions
What does T banding show?
Telomeric banding after heat denaturation
What is the G-banded chromosome band resolution?
What phase do chromosomes need to be for G-banding?
How is chromosome painting done?
Fluorescent-tagged chromosome specific DNA sequences.
What is aneuploidy?
the loss or gain of chromosomes
What is polyploidy?
Those containing more than 2 complete paired homologous sets of chromosomes
What is an ex of aneuploidy?
What is an ex of polyploidy?
Chromosome rearrangements alter
What are the 4 types of rearrangements?
What does inversion mean?
a segment of chromosome is turned 180