3.10-3.11 Molecular Biotech Flashcards Preview

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Flashcards in 3.10-3.11 Molecular Biotech Deck (11)
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1
Q

Know the basics of gel electrophoresis

A

DNA has a negative charge so it will migrate toward the + electrode

2
Q

How is LacZ used in cloning

A

Bac colonies that contain LacZ will be blue wen grown in media containing X-gal. When lacZ is interrupted by an inserted DNA fragment the colonies will be white

3
Q

How is LacZ used in cloning

A

LacZ can be used to screen bac containing recombinant plasmids

4
Q

What are the basics of PCR

A

DNA template, DNA pol enzyme, primers, nucleotides, and reaction buffer

5
Q

What are the weaknesses and strengths of PCR

A

Weaknesses: Prior knowledge of at least part of the seq of the target DNA. Capacity of PCR to amplify small amounts of DNA, Taq cant proofread. Strengths: Amplify small amounts of DNA from crime scenes. Identify genetic variation. Isolate DNA from ancient sources.

6
Q

What are the basics of quantitative PCR

A

probes with dif spectral wavelengths can be used to allow the quantitation of multiple targets in a single reaction

7
Q

What is chromosome walking and jumping

A

Walking: sequencing fragments from a point near to a gene of interest. Jumping: large fragment is circularized and the junction is sequenced

8
Q

What is positional cloning

A

isolating genes on the basis of their position on a genetic map

9
Q

How is positional cloning used to clone the CF gene

A

CF was located by positional cloning

10
Q

What is a humanized animal model

A

animal model that carries functional human genes cells tissues and or organs

11
Q

What is the basic concepts of CRISPR and Cas system of gene editing

A

CRISPER spacers recognize and cute exogeneous genetic elements by delivering the Cas9 protein and appropriate guide RNAs into a cell the target organisms genome can be cut at any desired location