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Flashcards in DNA replication Deck (17)
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1

What is responsible for removing erroneously placed nucleotides?

3'-5' exonuclease

2

Polymerases have a P and E site. What is their respective roles?

P site: polymerization
E site: editing

3

What is the role of MutS and MutL?

Detects mismatched base, cuts at it and at the next nick of the sequence

4

Define DNA polymerase I

fills the gaps during DNA repair

5

DNA polymerase II role

Repairs fork stalled by DNA damage

6

DNA polymerase III role

DNA replication

7

How long are RNA primers

10 nucleotides

8

DNA helicase role

Unwinds DNA

9

Clamp role

Keeps DNA polymerase on the strand synthesizing

10

Association between DNA helicase and DNA primase is called...

A primosome

11

Role of DNA topoisomerase

Makes temporary breaks in DNA backbone to relieve stress of unwinding

12

DNA primase in eukaryotes is made of a complex of subunits named....

DNA polymerase alpha/primase

13

What DNA polymerase is used on the leading strand?

epselon

14

What DNA polymerase is used on the lagging strand?

delta

15

What is the last two dimers to be added to the histone, therefore the first to come off in destabilization?

H2A-H2B dimer

16

What enzyme loads H2A-H2B dimer back into the nucleosomes?

NAP-1

17

What enzyme loads H3-H4 dimer back into the nucleosomes?

CAF-1