Flashcards in Control of gene expression in eukaryotes Deck (19)
Define the transcriptome
the mRNA complement of the genome expressed under certain conditions
Define the proteome
The protein complement of the genome expressed under certain conditions
What is the main characteristic of the transcriptional level?
controls the transcription initiation frequency of a gene
What is the main characteristic of mRNA maturation level?
Quality control, splicing
What is the main characteristic of mRNA transport and localization?
distribution to cell compartments
Define mRNA stability level?
Define translation level
control of mRNA decoding
Define protein activity level
folding, localization, modification, half-life
What are the three cis-acting regulatory elements?
Core promoter, proximal and distal regulatory elements
What is contained in the contents of the basal promoter?
contains the TATA box, 25-35 bp upstream from transcription initiation site. Remove the promoter, the RNA polymerase can't bind
Where are regulatory elements located?
They are located mostly upstream, but can also be found downstream
Regulatory DNA Binding Protein
Define the proximal reulatory element
1. located int eh first 350 bp of 5'-flanking region
2. activity is dependent upon their position and orientation
3. can increase, decrease, or inhibit transcription initiation frequency
Define the distal regulatory elements
1. located in 5', 3', or within introns
2. can work over large distances
3. activity is independent of position or orientation
4. can regulate entire chromatin domains
Define the locus control region (LCR)
DNA sequence elements that control the expression of one or several genes located within a large chromatin domain
How does the LCR work?
RDBPs bound to LCR interact with RDBPs bound to proximal regulatory elements of individual genes, initiating translation and decondensing chromatin via CRCs.
Define insulator elements
1. protect active genes by preventing heterochromatin formation.
2. Serve as physical barriers that limit action of distal regulatory elements
Why is the major groove frequently where RDBPs bind?
more diverse opportunities for binding and interaction