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Flashcards in Control of gene expression in eukaryotes Deck (19)
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1

Define the transcriptome

the mRNA complement of the genome expressed under certain conditions

2

Define the proteome

The protein complement of the genome expressed under certain conditions

3

What is the main characteristic of the transcriptional level?

controls the transcription initiation frequency of a gene

4

What is the main characteristic of mRNA maturation level?

Quality control, splicing

5

What is the main characteristic of mRNA transport and localization?

distribution to cell compartments

6

Define mRNA stability level?

Transcript half-life

7

Define translation level

control of mRNA decoding

8

Define protein activity level

folding, localization, modification, half-life

9

What are the three cis-acting regulatory elements?

Core promoter, proximal and distal regulatory elements

10

What is contained in the contents of the basal promoter?

contains the TATA box, 25-35 bp upstream from transcription initiation site. Remove the promoter, the RNA polymerase can't bind

11

Where are regulatory elements located?

They are located mostly upstream, but can also be found downstream

12

Define RDBP

Regulatory DNA Binding Protein

13

Define the proximal reulatory element

1. located int eh first 350 bp of 5'-flanking region
2. activity is dependent upon their position and orientation
3. can increase, decrease, or inhibit transcription initiation frequency

14

Define the distal regulatory elements

1. located in 5', 3', or within introns
2. can work over large distances
3. activity is independent of position or orientation
4. can regulate entire chromatin domains

15

Define the locus control region (LCR)

DNA sequence elements that control the expression of one or several genes located within a large chromatin domain

16

How does the LCR work?

RDBPs bound to LCR interact with RDBPs bound to proximal regulatory elements of individual genes, initiating translation and decondensing chromatin via CRCs.

17

Define insulator elements

1. protect active genes by preventing heterochromatin formation.
2. Serve as physical barriers that limit action of distal regulatory elements

18

Why is the major groove frequently where RDBPs bind?

more diverse opportunities for binding and interaction

19

Define the functional anatomy of all RDPBs

1. DNA binding site responsible for finding appropriate sequence
2. protein-protein interaction domains that influence the transcriptional activity of the target gene