Flashcards in DNA recombination Deck (17)
Define general recombination
switching that occurs between pairs of homologous sequences
Define RecA protein
used for synapsis of a single strand of DNA into a dsDNA
What are two homologues for RecA in eukaryotes that you should know??
Rad51 and Dmc1
the process of cutting the Holliday junction is called
What are the two enzymes that cut ds DNA in homologous recombination
Spo11 and Mre11 nuclease complex
What is a site of gene conversion?
where one strand was present in the middle of another gene
What is a Site of crossover?
Where heteroduplex forms between two joined strands of dsDNA
What is an example of DNA-only transposons?
What is an example of a retroviral-like retrotransposon?
What is an example of nonretroviral retrotransposons
What enzyme is required for DNA transposons
What enzymes are required for retroviral-like retrotransposons
reverse transcriptase and integrase
What enzymes are required for nonretroviral retrotransposons
reverse transcriptase and endonuclease
Describe the ends of a DNA transposon
short inverted repeats at the end
Describe the ends of a retroviral-like retrotransposons
directly repeated long terminal repeats (LTRs) at each end
Describe the ends nonretroviral retrotransposons
Poly A tail at 3' end of RNA transcript, 5' end often shortened