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Flashcards in DNA recombination Deck (17)
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1

Define general recombination

switching that occurs between pairs of homologous sequences

2

Define RecA protein

used for synapsis of a single strand of DNA into a dsDNA

3

What are two homologues for RecA in eukaryotes that you should know??

Rad51 and Dmc1

4

the process of cutting the Holliday junction is called

resolution

5

What are the two enzymes that cut ds DNA in homologous recombination

Spo11 and Mre11 nuclease complex

6

What is a site of gene conversion?

where one strand was present in the middle of another gene

7

What is a Site of crossover?

Where heteroduplex forms between two joined strands of dsDNA

8

What is an example of DNA-only transposons?

P-element (Drosophila)

9

What is an example of a retroviral-like retrotransposon?

Ty1 (yeast)

10

What is an example of nonretroviral retrotransposons

L1 (human)

11

What enzyme is required for DNA transposons

transposase

12

What enzymes are required for retroviral-like retrotransposons

reverse transcriptase and integrase

13

What enzymes are required for nonretroviral retrotransposons

reverse transcriptase and endonuclease

14

Describe the ends of a DNA transposon

short inverted repeats at the end

15

Describe the ends of a retroviral-like retrotransposons

directly repeated long terminal repeats (LTRs) at each end

16

Describe the ends nonretroviral retrotransposons

Poly A tail at 3' end of RNA transcript, 5' end often shortened

17

What is the difference between CRE and LoxP?

Cre is a recombinase protein. LoxP is the sequence Cre cuts at.