Mycobacterium Flashcards

1
Q

What are M

A

intracellular Bb - survive in infected cell

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2
Q

Where can the obl. pathogens only be found

A

in infected cells

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3
Q

Where can the fac. path and saprphytes be found

A

in the environment, soil, water, plants

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4
Q

What is the morph of M

A

rods, coccoid difference in cell structure in different species

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5
Q

What’s the resistance like

A

excellent

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6
Q

Staining

A

gram + but gram often doesn’T work

Ziehl Neelsen +

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7
Q

O2

A

obl aerobic

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8
Q

Culture

A

broth: Henley, Sauton
solid: egg, serum, agar
long inc.

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9
Q

Antigens

A
a lot
Glycolipids, lipoproteins:
-shared
-cross reactions
tuberculin
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10
Q

Special test for tuberculin

A

PPD (purified protein derivate) basically a transdermal injection of tuberculin and if a person had been subjected to it before AB reaction would show

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11
Q

!Name all the obl. pathogens in M and their possible hosts! (10)

A
M. tuberculosis - Ho, Su, Ca,Fe,Parrot, RU
M.africanum - Ho 
M.Bovis - Ru, ru, Su, Ca, Fe, Ho
M.caprae - Ru, ru, Su, Ho
M.microti - rodents
M.avium ssp avium - birds
M.avium ssp paratuberculosis - Ru, ru
M.avium ssp silvaticum - Av, Ru
M.leprae - Ho
M.lepraemurium - Ho, mouse
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12
Q

How are M. leprae, lepraemurium special

A

well they cause and also they’re parathrophic meaning they need living cells and cannot be cultured on synthetic medium

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13
Q

How do ZN + Bb stain

A

red

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14
Q

How do ZN - Bb stain

A

blue

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15
Q

What is the order of ZN staining

A

fixed - carbol-fuchsin - acid and alkohol - methylene blue

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16
Q

How long is the inc. period for M.tuberculosis

A

3-6 w

17
Q

! Name all the fac pathogens !

A
M. fortuitum
M. marinum
M. kansasii
M. scrofulaceum
M. gordonae
M. intracellulare 
M. terrae
M. phlei
18
Q

How can you group the fac pathogens

A

photochromogens - light needed
scotochromogens - light is not needed
non-chromogens - can’t produce pigment
fast growers - grow in under one week

19
Q

Photochromogens

A

M.kansasii, M.marinum

20
Q

What do M.fortuitum and M.marinum cause

A

fish and reptile tuberculosis

21
Q

Scotochromogens

A

M. scrofulaceum, M.gordonae

22
Q

Non-chromogens

A

M.intracellulare, M.terrae

23
Q

Rapid growers

A

M. fortuitum, M.phlei

24
Q

What do the fac. pathogens cause

A

local lesions Su, Ru

25
Q

Which Bb can cause paratuberculosis and what is it

A

M. avium ssp. paratuberculosis, M.avium ssp silvaticum (Ru)

-paratuberculosis - weight loss, diarrhea