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Vet. Bacteriology > Enterobacteriaceae > Flashcards

Enterobacteriaceae Flashcards

1
Q

What kind of taxonomic unit is Enterobacteriaceae

A

Family

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2
Q

What other subunits are in Enterobacteriaceae

A

ca. 40 genera

ca. 180 species

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3
Q

where to find Enterobacteriaceae Bb

A

normal gut flora

through faeces into the environment

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4
Q

Most of them are pathogenic T/F

A

F most are saprophytes - can be helpful

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5
Q

Morphology

A

1-3 micrometer rod

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6
Q

All of them have flagella T/F

A

F all of them except shigella and klebsiella

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7
Q

Klebsiella and Shigella

A

have no flagella

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8
Q

Resistance

A

average

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9
Q

Staining

A

Gr -

red pinkish

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10
Q

Culture

A

simple aerobic, fac. anaerobic
10-45°c
1 day to produce colony

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11
Q

What is used for differentiation mainly

A

lactose fermentation

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12
Q

name a selective enrichment for Salmonella

A

Rambach agar

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13
Q

What do the differential media contain

A

lactose, indicator and inhibitory materials

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14
Q

What’s in a low selective capacity medium and what can grow in it

A

all kind of enterobacteriaceae and some Gr+ can grow
crysatl violet - lactose - litmus
crystal violet - bile salts - lactose - neutral red

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15
Q

What’s in a medium selective capacity medium and what can grow in it

A

only the most important enterobacteriaceae can grow

brilliant green - lactose - phenol red - E. and S.

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16
Q

High selective cap.

A

designed for isolation of salmonella

-bismuth sulphite agar

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17
Q

O/C

A

catalase +

oxidase -

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18
Q

O/F test

A

fermentative (acid + gas prod)

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19
Q

What else can be used for differentiation

A

the antigens

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20
Q

What are the antigens for differentiation

A

O, K, H, F

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21
Q

What do the antigens stand for

A 
O = cell wall
K= capsule
H= flagella
F= fimbira
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22
Q

Name the lactose pos. genera

A
  • Escherichia
  • Klebsiella
  • Enterobacter
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23
Q

Name the lactose neg. genera

A
  • Salmonella
  • Shigella
  • Yersinia
  • Proteus
  • Providencia
  • Edwardsiella
  • Serratia
  • Citrobacter
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24
Q

Most of Enterobacteriaceae are fac path T/F

A

F overwhelming majority is saprophytic

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25
Q

Where are KLebsiella found

A

gut, resp. tract, Genital tract, environment, muc mem

26
Q

Morphology of klebsiella

A

rod, capsullated, non flagellated

27
Q

Describe the Klebsiella colonies

A

large merging colonies extensive capsule

fast metabolism

28
Q

Enterobacteriaceae diff.

A

lactose +
Voges proskauer +
urease +

29
Q

Name the relevant species of Klebsiella

A

K.pneumoniae

30
Q

List the diseases a fac. K. pneumoniae could cause

A

Calf: bronchitis, bronchopneumonia
Cow: mastits
Mare: metritis
Ho: pneumonia, urogenital infections

31
Q

Where to find Enterobacter

A

gut, environment

32
Q

Morphology of enterobacter

A

rod, thiccccccc capsule

33
Q

Enterobacteriaceae diff

A

lactose +

Voges proskauer test+

34
Q

Enterobacter is largely saprophytic T/F

A

T

35
Q

What are the G- anaerobic rods

A

Fusobacterium, Bacteroides and Dichelobacter

36
Q

Where to find the G- anaerobic rids

A

muc mem, alimentary tract, resp. tract, genital tract

37
Q

Morph of Fusobacterium

A

polymorph

38
Q

Culture of Fusobacterium

A

fastidious, obligate anaerobic, CO2

39
Q

Species of Fusobacterium

A

F.necrophorum
-ssp necroforum
-ssp funduliforme
F.nucleatum

40
Q

What is the morphology of F.necrophorum

A

long chains, filaments

41
Q

Culture of F.necrophorum

A

blood agar, obl. anaerobic, haemolysis

42
Q

What diseases does F. necrophorum cause

A

ssp. necroforum causes Necrobacillosis in calf, piglet, rabbit
and
foot rot in sheep
ssp funduliforme is saprophytic

43
Q

What diseases does F.nucleatum cause

A

human carnivore abscess, mastitis, metritis

44
Q

Morphology of bacteroides

A

3-5 micrometer rod with fimbria

45
Q

Culture of bacteroides

A

fastidious , obl anaerobic

46
Q

What’s the most important spec. in Bacteroides

A

B.nodosus

47
Q

What does B. nodosus cause

A

foot rot in sheep

48
Q

What does Dichelobacter nodosus cause

A

foot rot in sheep

49
Q

Where to find escherichia coli

A

gut, muc mem

50
Q

Morphology of E.coli

A

2-3 mircom rod with capsule thiccc

51
Q

Is E.coli flagellated

A

Yes

52
Q

E-coli ha fimbria

A

Oh yeah

53
Q

Culture of E.coli

A

aerobic, fac. anaerobic
simple nutrient agar or broth
colonies appear very fast

54
Q

Enterobacteriaceae diff

A

lactose +

indol + (indol prod. from tryptophane)

55
Q

list all the antigens of E.coli

A

O: type specific (174 types)
K: heat stabile (A) and heat labile (L) : 80 types
F: 19 types
H:57 types

56
Q

Which F antigens belong to which species

A 
F1: common fimbria
F2,F3: human
F4,F6,F18:Su
F5,F17,F41: Ru
F7-16: UTI Ho and poultry
57
Q

What can E.coli cause in Cattle

A

calf coli diarrhoea and septicaemia, cow mastitis metritis

58
Q

What can E.coli cause in Su

A

neonatal pigs: coli-diarrhoea
weaned piglets: coli-diarrhoea, edema disease
Sow: mastitis, metritis

59
Q

What can E.coli cause in lamb

A

Coli-diarrhoea

60
Q

What can E.coli cause in poultry

A

dayoldchick: septicaemia
chicken: septicaemia
older: lesions in organs

61
Q

What can E.coli cause in Ho

A

neonatal enteritis, septicaemia, urogenital infections, enteritis, haemorrhagic enterocolitis

62
Q

!What protein do pathogenic strains of E.coli produce!

A

Intimin - attaches to surface of enterocytes and destroys them
Enterotoxin - makes enterocytes excrete causing diarrhoea
Necrotoxic - attack epithelial gut cells

Vet. Bacteriology (12 decks)

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