Follicular lymphoma molecular
Primary cutaneous follicle center lymphoma (PCFCL) vs nodal follicular lymphoma
Most cases PCFCL lack t(14;18) are Bcl6 pos, but Bcl2 and CD10 neg Vs in lymph node FL has t(14;18) and is pos for BCL6, BCL2 and CD10
B-cell markers: CD19, CD20 (*dim), CD22
Neg for CD10 Bcl6- FMC-7
*CD38 pos and ZAP-70+=bad prognosis
Immunophenotype mantle cell lymphoma
B-cell markers: CD19, CD20 (bright), CD22, FMC-7
CD5+ CD23- Cyclin D1+ CD43+
Immunophenotype of follicular lymphoma
CD10+ Bcl6+ Bcl2+ (90%)
Immunophenotype of marginal zone lymphoma
B-cell markers; nothing specific
Which two lymphomas express CD25
Hairy cell leukemia (also CD20, CD22, TRAP)
Adult T cell leukemia/lymphoma
What lymphoma shows T-cell rosettes?
NLP hodgkin lymphoma CD3+ CD57+ T cells surround LP cells forming rosettes
Burkitt lymphoma translocation
Which 3 lymphomas express CD30?
1. RS cells in HL
3. mediastinal B-cell lymphoma
What distinguishes plasmablastic lymphoma from other plasma cell neoplasms?
Flow findings in PNH
-RBCs show loss of CD55 and CD59
-Granulocytes show loss of CD16 and CD24
Which BCR-ABL isoform is associated with CML?
p210 isoform of t(9;22) BCR-ABL
Which BCR-ABL isoform is associated with B-ALL?
p190 isofrom of t(9;22) BCR-ABL
What subtype of AML is associated with thrombocytosis and giant agranular platelets?
AML with inv3
Translocation in acute monocytic leukemia
t(8;16) blasts with characteristic cytoplasmic vacuoles
Blasts w/ characteristic vacuoles
acute monocytic leukemia t(8;16)
Favorable prognostic factors B-ALL
Poor prognostic factors B-ALL
t(11q23) MLL rearrangement
What B-cell neoplasm shows clumpy "soccer ball" chromatin on peripheral smear and cytology?
What translocation is found in mantle cell lymphoma
results in cyclin D1 amplification
FISH is best assay
Marginal zone lymphoma
"monocytoid" lymps w/ abundant clear cytoplasm
Immunophenotype of hairy cell leukemia
B-cell markers: CD19, CD20, CD22
CD11c (bright) and CD25 (bright)
cyclin D1 (dim nuclear)
most common lymphomas in HIV infection
DLBCL (esp CNS)
Primary effusion lymphoma
, plasmablastic lymphoma
Subtype of B-ALL/LBL with peripheral blood eosinophilia
What type of lymphoma typically presents as an anterior mediastinal mass often with hypercalcemia?
Good prognosis in CLL/SLL
normal karyotype or del13q only
mutated status of Ig heavy chain
Bad prognosis in CLL/SLL
-del11q or 17p
ZAP70 and/or CD38 expression
lack of somatic hypermutation