Flashcards in TM Deck (72)
How many platelets in a pheresis unit?
Minimum donor intervals RBC
>=8 weeks for single unit whole blood donation
16 weeks if double unit by apheresis
Lacks H and secretor genes (hh, sese)
What sugar makes A antigen
What sugar makes B antigen?
what minor red cell antibody is most common cause of ABO discrepancy?
*Redo ABO testing with M neg cells
What infection associated with Anti-I autoantibody?
What infection associated with anti-i autoantibody?
PCH caused by what antibody?
Most common Weiner haplotype in caucasians?
Most common Weiner haplotype in african americans?
duffy negative confers resistance to what?
3 possibilities of RhD+ person showing anti-D?
1. Variant/partial D (weak D testing will pick up)
2. Auto-antibody (DAT +)
3. Anti-LW antibody (DAT -)
Which antibodies show dosage
Kids, Monkeys (Rh) and MeN Show Dosage
-->Kidd, Rh, MNS and Duffy
Which antibody shows anamnestic response?
Kidd system (kids hide)
Which antibodies are enhanced by enyzmes?
Give sugar (CHO antigens) to Kids and monkeys (Rh) and they will jump
Le, I, P, ABO
Which antibodies are decreased by enyzmes?
Daffy MeNS Lutheran Choir falls down
Which antibodies are unaffected by enyzmes?
Kell (doesn't care)
RBC/Whole blood storage
21 days (CPD/2D)
35 days (CPDA-1)
42 days (AS_
Frozen RBC storage
10 yrs @-65C
24 hrs @1-6C after thaw
Washed RBC storage
24 hrs @1-6 C
5 days @20-24 C (gentle agitation)
4 hours if pooled in open system
24 hrs 20-24C (no agitation)
1 year @-18C
7 years @ -65C
24 hrs at 1-6 C after thaw
1 year @-18C
6 hrs @20-24C after thaw (4 hours in pooled open system)
RBC quality control
HCT <80% all
>50g Hb in 95% (apheresis RBC)
Quality control leukoreduced RBCs
<5x10^6 WBCs in 95%, retain 85% of RBCs
Quality control cryo
factor 8>80IU, fibrinogen >150 mg
RBC storage lesion
Decreased 2,3 DPG
Decreased K+ in red cells, but increased K+ extracellularly