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Flashcards in Prof's Review ques Deck (24)
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1

WHO top 10

Acute respiratory infections - 3.5 mn
Diahhreal diseases - 3.1 mn
AIIDS - 1.8 mn
TB -1.3 mn.
Malaria 0.66. mn.

2

who developed the rabies vaccine -

louis pasteur

3

what is a genus

2 or more species with similar morphology

4

what is a genus

group of organisms with similar genetics

5

what is typical size for humans??

1.6 m

6

typical size of bacterium?

1-5 micro meter

7

typical size of virus?

50-250 nanometer

8

morphology?

rod shaped = bacillus
sherpical or round - coccus
curve or wavy - curved rod = vibrio
flexible wave - spirochete
rigid wave - spirilum

9

simple stain

stain all bacteria same color
crystal violet

10

Difference between spirochete and spirilum

spirochete - flexible wave
spirilum - rigid wave

11

Gram stain (+ve) - S.Aureus

purple -->blue purple (after adding iodine)-->remains blue purple -->(after alcohol wash) -->remains blue purple -->(after adding sarfarin)

12

Gram stain (-ve) E.Coli

purple -->blue purple (after adding iodine)-->looses stain -->(after alcohol wash) -->orange red -->(after adding sarfarin)

13

which staining technique is most effective?

gram stain

14

Gram +ve cell structure

thicker cell wall and thin cell membrane
thicker cell wall structure makes it nonrespondent to alcohol

15

Gram -ve cell structure

outer membrane
cell wall
cell membrane

have two membranes but are very thin and allows the alcohol to penetrate and wash the stain

16

what is gram +ve susceptible to?

penicillin (only thing that can destroy the cell wall)

17

gram -ve susceptible to?

tetracycline

18

Acid fast stain

used to identify mycobacteria (TB and leprosy)

19

Mycobacteria

obligate

20

key differences between gram +ve and -ve

POSITIVE
-thicker peptidoglycan
-no outer membrane
-teichoic acid
NEGATIVE
-thinner peptidoglycan
-outer membrane persent (periplasmic region)
-LPS (endotoxin)

21

steps of animal virus replication

fusion uncoating and budding

22

steps of viral growth and replication

attachment, penetration,uncoating,biosynthesis,maturation and release

23

steps that are missing in viral replication

fusion and budding

24

which diseases are caused by prions

fatal neurological diseases (deer, elk, CWD)
sheep and goats (scrapie)
cows (bovine,spongiform, encephalopathy, BSE)
humans (CJD)