Cell Biology. Flashcards

1
Q

What are all things made from?

A

Cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the two types of cells?

A

Prokaryote and eukaryote.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Describe an eukayote cell.

A

Complex and include animal and plant cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Describe a prokaryote cell.

A

Smaller and simpler - Bacteria.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the scientific word for the different parts of a cell?

A

Subcellular structures.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Name the sub-celluar structures of an animal cell.

A

Nucleus Cytoplasm Ribosomes Cell Membrane Mitochondria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Define a nucleus.

A

Contains genetic material that controls the activities of the cell.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Define a cytoplasm.

A

gel like substance where most of the chemical reactions happen. It contains enzymes that control these reactions.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Define cell membrane.

A

Holds the cell together and controls what goes in and out.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Define mitochondria.

A

these are where most of the reactions for aerobic respiration takes place. Respiration transfers energy that the cell needs to work.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Define Ribosomes.

A

these are where the proteins are made in the cell.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Give the 3 subcellular structures of a plant cell.

A

Cell Wall Permananent Vacuole Chloroplasts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Define a cell wall of a plant.

A

Rigid cell wall made from cellulose it supports the cell and strenghtens it.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Define a permanent vacuole.

A

contains cell sap a weak solution of sugars and salts.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Define the chloroplasts of a plant.

A

where photosynthesis occurs which makes food for the plant. They contain a green substance called chlorophyll, which absorbs the light needed for photosynethesis.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What does a bacterial cell not have?

A

chloroplasts, mitochondria and don’t have a true nucleus instead they have a single circular strand of DNA that floats freely in the cytoplasm.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Sub-cellular structures of a bacteria.

A

Cell-membrane Cytoplasm Cell wall Plasmids-small rings of DNA. Circular strand of DNA thats floats freely in the cytoplasm.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What do microscopes allow us to do?

A

see things we can’t see with the naked eye. And microscopy techniques hae developed over the years as technology adn knowledge has improved.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What are light microscopes?

A

Use light and lenses to form and image of a specimen and magnify it. Let us see individual cells and large subcellular structures like nuclei.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What is an electron microscope?

A

use electrons to form an image.Thye have higher magnification and a higher resoloution (ability to distinguish between two points - a sharper image) you can see smaller things in more detail like the internal structure of a chloroplast and ribosomes and plasmids.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Formula for magnification?

A

image size / real size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Magnification of triangle.

A

Image size mag real size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

How to prepare a slide to iew onion cells.

A

Add a drop of water to the middle of a clean slide. Cut up an onion and seperate it out into layers. Use tweezers to peel off some epidermal tissue from the bottom of one of the layers. Using tweezers place the epidermal tissue into the water on the slide. Add a drop of iodine soloution iodine solution is a stain stains are used to highlight objects in a cell by adding colour to them. Place a cover slip on top. To do this stand the cover slip upright on the slide next to the water droplet. Then carefully tilt and lower it so it covers the specimen. Try not to get any air bubbles under there as theyll ebstruct the view of the specimen.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

How to look at your slide.

A

Clip the slide you have prepared onto the stage. Select the lowest powered objective lens Use the coarse adjustment knob to move the stage up to just below the objective lens. Look down at the eyepeice. use the coarse adjustment knob to move the stage downwards until the image is roughly in focus. Adjust the focus with the fine adjustment knob until you get a clear image of whats on the slide. If you need to see the slide with greater magnification swap to a higher powered objective lens and refocus.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

How to draw your obserations from a microscope.

A

Draw what you see under the microscope using a pencil with a sharp point. Make sure drawing takes up at least half of the space and is drawn clearly with unbroken lines. Your drawing should not inculde any colour or shading. When drawing cells the subcellular structures should be drawn in proportion. Remember to include a title of what your’e observing and write down the magnification that it was observed under. Label the important features of your drawing using straight uncrossed lines.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

What is differentiation?

A

process by which a cell changes to become specialsised for its job. As cells change they develop different subcellular structures and turn into different types of cells which allows them to carry out specific functions.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

When does most differentation occur.

A

As an organism develops

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

When does differentiation occur in an animal cell?

A

Is lost at an early stage after they become specialised however lots of plants cells dont ever lose this ability.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

What are differentiated cells in mature animals used for?

A

Repairing and replacing cells such as skin or blood cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

What are differentiated cells called?

A

Stem cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Examples of specialised cells.

A

Sperm Cells Nerve Cells Muscle Cells Root hair cells Phloem and Xylem cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

What are sperm cells specialised for?

A

reproduction, the function of a sperm is to get male DNA to the female DNA.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

How has sperm adapted?

A

It has a long tail and a streamlined head to help it swim to the egg. There are lots of mitochondria in the cell to provide the energy needed. It also carries enzymes in its head to digest through the egg cell membrane.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

What are nerve cells specialised for?

A

Rapid signalling.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

How have nerve cells adapted?

A

the function of nerve cells are carrying electrical signals from on part of the body to another. These cells are long to cover more distance and have branched connections at their ends to connect to ther nerve cells and form a network throughout the body.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

What are muscle cells specialised for?

A

contraction.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

How have muscle cells adapted?

A

the function is to contract quickly. These cells are long so they have space to contract and contain lots of mitochondria to generate the enrgy needed for contraction.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

What are root hair cells specialised for?

A

absorbing water and minerals.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

How have root hair cells adapted?

A

root hair cells are on the surface of plant roots which grow into long hairs that stick out into the soil. This gives the plant a big surface area for absorbing water and mineral ions from the soil.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

What are phloem and xylem cells specialised for?

A

transporting substances

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

How are phloem and xylem adapted?

A

Form phloem and xylem tubes which transport substances such as food and water around plants.

To form these tubes the cells are long and join at the ends.

Xylem cells are hollow in the cnetre and phloem have few subcellular sructures so stuff can flow through them

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

Where are chromosomes?

A

most cells in your body have a nucleus, the nucleus contains genetic material in the form of chromosomes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

What are chromosomes?

A

coiled up lenghts of DNA molecules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

What does each chromosome carry?

A

large number of genes different genes control the development of different charachterisitics eg:hair colour

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

What do body cells have two copies of?

A

each chromosome one from the organisms mum and dad

so humans have two copies fo chromosome 1 chromosome 2 etc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

How many pairs of chromosomes in a cell?

A

23

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

What is the cell cycle?

A

body cells in multicellular orgainsm divide to poduce new cells as part of a series of stages in the cell cycle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

What is the stage of the cell cycle when the cell divides?

A

mitosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

What is mitosis for?

A

grow or replace cells that have been damaged

50
Q

What the end of the cell cycle results in?

A

two new cells identical to original one with same number of chromosomes

51
Q

describe the growth and dna replication part of mitosis

A
  • in cell thats not dividing, the DNA is spread in long strings
  • befor divides the cell grows and increases the amount of subcellular strutures ex:mitochondria and ribosomes
  • it then duplicates its dna
  • so theirs one copy of each new cell
  • dna is copied then forms x shped chromosomes
  • each arm of chromosome is exact duplicate of the other
52
Q

Describe mitosis

A
  • once contents and dna is copied the cell is ready for mitosis
  • chromosomes line up at centre of cell and cell fibres pull them apart
  • two arms of each chromosome go to opposite ends of cell
  • membranes form around each sets of chromosomes
  • these become the nuclei of the two new cells the nucleus had now divided
  • lastly cytoplasm and cell membrane divide
  • the cell has now produced two new daughter cells
  • the daughter cells contian exactly the same dna
  • identical
  • their dna is also identical to their parental cell
53
Q

How prokaryotic cells reproduce?

A

binary fission

54
Q

Describe the process fo binary fusion

A
  • ciruclar dna and plasmids replicate
  • cell gets bigger and circular dna strands move to opposite poles of the cell
  • cytoplasm divides and new cell walls form
  • cytoplasm divides and 2 daughter cells are produced
  • each daughter cell has one copy of cirlcular dna but can have a varible number of copies of plasmids
55
Q

When do bacteria divide quickly?

A
  • right conditions warm environment and right nutrients
  • some bacteria eg:E-Coli take 20min to replicate if enviroment is right
56
Q

What happens is conditions are unfavourable?

A

cells stop dividing and will die

57
Q

How to find the number of bacteria in a population?

A
  • mean division time is average amount of time it akes for one bacterial cell to divide into 2.
  • if you know this you can work out how mnay times it has divided in a certain amount of time and the number of cells produced in that time
  • to find out the cells it would produce:
  • make sure the untis are the same
  • divide the two
  • put this answer to the power of 2 so (…)^2
58
Q

Where is bacteria grown?

A
  • in a cultured medium
  • conrians carbodhydrates minerals and vitamins to grow
  • culture medium used can be a nutrient broth solution or solid agar jelly
  • Bacteria grown on agar plates will form on visible colonies on the surface of jelly or will spread out to give even covering of bacteria
59
Q

How to make a agar jelly plate?

A
  • hot agar jelly poured into shallow round dush called petri dishes
  • when jelly cool and set inocualting loops can be used to transfer microorganisms to culture medium
  • or sterile pippete and spreader can be used to get an even covering of bacteria
  • microorganisms then multiply
60
Q

Why arent cultures of microorganisms not kept at above 25?

A
  • harmful pathogens are more likely to grow above this temperature
  • in industrial conditions cultures are incubated at higher temps so they grow faster
61
Q

How to test the actions of antibiotics on cultures of bacteria?

A
  • place paper disk soaked in different types or concentrations of antibiotics on an agar plate that has an even covering of bactria.
  • leave some space between discs.
  • antibitic will diffuse into the agar jelly
  • antibiotic resistance bacteria will grow on agar around the paper discs but non resistant strains will die
  • a clear area will be left where bacteria have died this is called inhibition zone
  • The control= paper disc not been soaked in antibiotic
  • instead soak in sterile water
  • then you can be sure difference between growht of bacteria around the control disk and antibiotic disk due to antiobiotic alone and not defect in paper
  • leave plate for 48 hours at 25 degrees
  • more effective antibiotic is against the bacteria the larger inhibition zone will be
62
Q

What will contaminated instruments cause?

A

affect results and result in growth of pathogens

63
Q

How to avoid contaminated cultures?

A

Petri dish and culture medium is sterilised before used by heating to high temp

to kill inwanted microorganisms

if innoculating loop is used to transfer bacteria to culture medium sterilise first by passing throough hot flame

after bacteria transfer lid of petri dish should be tightly on to stop microragnisms from air getting in

petri dish shoud be stored upside down to stop condensation falling on agar jelly

64
Q

How to compare the effectivness of antibiotics?

A
  • looking at relative sizes of the inhubiiton zones.
  • larger it is around the disk the more affective it is agiainst bacteria
  • you can do it by eye or by calclation
65
Q

How to calcualte area of zone of inhibition or the area of a colony?

A

Area = Pi x Radius ^2

66
Q

What is differentiation?

A

process by which a cell changes to beocme specialised for its job

67
Q

What are undifferentiated cells?

A

stem cells which divide to produce lots more undifferentiated cells.

they differentiate into different types of cells depending on the instructions they are given.

68
Q

Where are stem cells found?

A

early human embryos

69
Q

Why stem cells are exiting to scientists?

A

potential to turn into any type of cell

70
Q

Do adults have stem cells?

A

Yes, certain places like bone marrow.

Unlike embroyonic stem cells they cannot turn into any type of cells only certain ones like blood cells

71
Q

How are stem cells used?

A

grown in labs to produce clones and made to differentiate into specialised cells for medicine and research

72
Q

Does medicine already use stem cells?

A
  • yes
  • ex: stem cells from the bone marrow of healthy person replace faulty bloody cells in person receiving it
73
Q

What can you do with embryonic stem cells?

A

replace faulty cells in sick people

make insulin producing cells for diabetic or nerves cells for people paralysed

74
Q

What is therapeutic cloning?

A
  • embryo could be made to to have same gentic material as the patient
  • so stem cells produced from it would contain same genes and wouldn’t be rejected by patient is used to replace faulty cells
75
Q

Risks of stem cells?

A

stem cells grown in lab are contaminated with a virus which could be passed on to patient making them sicker.

76
Q

Why are people against stem cell research?

A
  • human embryos shouldn’t be experimented on since each one is potential huaman life
  • scientists should concentrate on finding and developing other sources of stem cells so people are cured without embryos
  • in some countries its banned in uk it is allowed bu follows strict guidlines
77
Q

For stem cells?

A
  • Curing patients is more important than rights of embryos
  • Embryos used in research unwanted ones from fertility clinics if they werent used for research would be destroyed
78
Q

Where are stem cells found in plants?

A

Meristems -part of the plant where growth occurs-

79
Q

When are meristems used?

A

through plants entire life cells in meristem tissues differntiate into any type of cell

80
Q

What meristems produce?

A

these stem cells can produce clones of whole plants quick and cheap

used to grow more plants of rare species to prevent wiping out

grow crops of identical plants that have desired features for farmers ie: disease resistance

81
Q

Define diffusion

A

Spreading out of particles from an area of higher concentration to an area of lower of concentration.

82
Q

Where does diffusion happen?

A

solutions and gases as the particles in these substances are free to move freely

83
Q

What is the simplest type of diffusion?

A

when gases diffuse through each other, for example when perfume diffuses through the air.

84
Q

How to increase the rate of diffusion?

A
  • bigger concentration gradient (difference in concentration)
  • a higher temperature because particles have more energy so move around faster.
85
Q

Why are cell membranes useful?

A

Hold the cell together but they let stuff in and out.

Dissolved substances can move in and out of cells by diffusion.

Only very small molecules can diffuse through the membrane eg: oxygen needed for respiration, glucose, amino acids, water

Big molecules like starch proteins can’t fit through.

86
Q

In diffusion of solution how does particles move across the membrane?

A
  • from a area of high concentration to an area of low concentration.
  • Move randomly so they will go both ways but if there are a lot more particles on one side of the membrane theres a net movement from that side
  • larger surface are of the membrane the faster diffusion rate as more particles pass through at once.
87
Q

Define osmosis

A

movement of water molecules across a partially permeable membrane from a region of higher water concentration to a region od lower water concentration

88
Q

what is a partialy permeable membrane?

A

very small holes in it

so small, only tiny molecules can pass through and bigger ones can’t (sucrose)

89
Q

Which way do water molecules pass?

A

both ways through the membrane during osmosis

as water molecules move around randomly

90
Q

What does it mean if there are more water molecules on one side than the other of a membrane in osmosis?

A

Steady net flow of water into region with fewer water molecules ie: into the stronger sugar solution which means it will get more dilute. the water acts like its trying to balance each side of the membrane

91
Q

Describe the practical which shows how osmosis works?

A

cut up potato in identical cylinders, and some beakers with differnt sugar solutions in them.

one of pure water, one very concentrated solution (1mol/dmcubed) then few others with concentrations between (0.2,0.4,0.6)

measure mass of potatoes then leave one cylinder of potato in each beaker for 24 hours.

then take them out, dry with paper towel, measure mass again

if potato has drawn in water by osmosis the mass will have increased, if drawn out it would decrease

calculate percentage change in mass then plot a graph

92
Q

Describe the variables of the osmosis experiment

A

dependant=chip mass

independant=concentration of sugar solution

all other variables (volume of solution, temp,time,type of suagr) must be kept the same or it is not a fair test

93
Q

Errors which could occur in osmosis experiment?

A

if some potato is not fully dried, excess water would have higher mass

If watr evaporated from beakers the concentrations of sugar solution would change

to reduce these effects by repeating the experiment,and calculate mean percentage change at each concentration or use different salt solutions to see effect on mass

94
Q

What is active transport?

A

substances are absorbed against a concentration gradient eg: from a lower concentration to a higher concentration

95
Q

How do root hair cells absorb minerals and water?

A
  • cells on plant roots grow into hairs which stick out of soil
  • each branch of a root are covered in millions of microscopic hairs
  • give the plant large surface area for absorbing water and mineral ions from soil
  • they need these ions for healthy growth
  • concentration of minerals is higher in root hair cells than the soil around them
  • so root hair cells can’t use diffusio to take up minerals and soils
96
Q

How roota hairs take up minerals from active transport?

A

minerals should move out of the root hairs if they followed rules of diffusion

cells must use another method to draw them in

this is active transport

it allows the plant to absorb minerals from a dilute solution against a concentration gradient.

this is essential for growth

bu active transport needs energy from respiration to work

97
Q

Where does active transport also happen?

A

In humans, in taking glucose from the gut and from the kidney tubules.

98
Q

How is active transport used in the gut?

A

where there is a lower concentrationof nutrients in the gut but a higher concentration in the blood

when theres a higher concentration of glucose and amino acids in the gut they diffuse naturally into the blood

but sometimes theres lower concentration in the gut than their is in the blood, so the concentration gradient is the wrong way

active transport allows nutrients to be taken from the blood despite the fact the concentration is the wrong way

so glucose is taken into bloodstream when its concentration in the blood is higher than in the gut. Then it is transported to cells for respiration.

99
Q

Give an example of how cells use diffusion to take in substance they need and get rid of waste products?

A

oxygen and co2 transferred between cells and environment during gas exchange

in humans urea diffues fri cells into blood plasma for removal from the bdoy by kidneys

100
Q

What depends on how easy it is for an organism to to exchange substances with its enviroment?

A

organisms surface area to volume ratio

101
Q

How to find surface area to volume ratio?

A

S.A=length x width then times by all the faces

Volume=length x width x height

102
Q

In a single celled orgainsm can gases and dissolved substances diffuse directly in or out of the cell across the membrane?

A

yes, as they have large surface area compared to volume

so enough substances can be exchanged across membrane to supply volume of the cell

103
Q

Problem with multicellular organisms?

A
  • smaller s.a compared to volume
  • not enough substances can diffuse from outside surface to supply entire volume
  • so they need exhange surface for efficeient diffusion
  • the exhange surface structures have to allow enough of the neccesary substances to pass through
104
Q

How are exhange surfaces adapted to maximise effectiveness?

A
  • thin membrane so substances only have a short distance to diffuse
  • large surface area so lots of substances diffuse at once
  • exchange surfaces in animals have lots of blood vessels to get stuff into/out of the blood quickly
  • gas exchange surfaces in animals are ventilated so air moves in and out
105
Q

How does gas exchange happen in the lungs?

A
  • job of the lungs is to transfer oxygen to the blood and to remove waste carbon dioxide from it.
  • To do this lungs contin millions of tiny air sacs called alveoli where gas exchange takes place
106
Q

How are the alveoli specialised to maximise the diffusion of co2 and o2?

A
  • enormous surface area 75m^2
  • mosit lining for dissolving gases
  • very thin walls
  • a good blood suply
107
Q

What is the inside of the small intestine covered in?

A

millions of tiny projections called villi

108
Q

What does villi do?

A

increase the surface area in a big way so that digested food is absorbed much more quickly into the blood

109
Q

How villi are specialised for their function of absorbing digested food?

A

single layer of curface cells

very good blood suply to assist quick absorption

110
Q

How does co2 diffuse into a plant?

A

Diffuses into the air spaces within the leaf then it diffuses into the cells where photosynthesis happens.

111
Q

Where is an exchange surface in a leaf?

A

Underneath a leaf.

Covered in stomata which co2 diffuses in through.

Oxygen and water vapour also diffuse out through stomata (water vapour is lost from all over the leaf surface ut most of it is lost through stomata)

112
Q

What is the size of stomata controlled by?

A

Guard cells, they close stomata is the plant is osing water faster than it is being replaced by the roots.

Without guard cells the plant would wilt.

113
Q

How does the leaf increases its area of exchange surface so it is more effective?

A

It is a flattened shape.

114
Q

Give another exhange surface in the leaf?

A

Walls of the cells inside the leaf

air spaces inside the leaf increases area of this surface so more chance of co2 getting into the cells

115
Q

What happens to water vapour inside the plant?

A

Evaporates from the cells inside the leaf.

Then escapes by diffusion as there is lots of it inside the leaf and less of it in the air outside

116
Q

What are gills?

A

Gas exchange surface for a fish.

117
Q

What happens to the water entering the fish?

A
  • It contains oxygen and enters through the mouth and passes out through the gills.
  • As this happens oxygen diffuses from water into the blood in the gills and co2 diffuses from blood into water.
118
Q

What is each gill made of?

A
  • Thin plates called gill filaments which give a big surface area for exchanging of gases
119
Q

What are the gill filaments covered in?

A

Tiny structures called lamellae which increase surface area even more.

120
Q

What does the lamellae have?

A
  • Lots of blood capilliaries which speed up diffusion.
  • Thin surface layer of cells to minimise distance gases have to diffuse
121
Q

Which direction does blood flow through the lamellae?

A

One direction and water flows over in the opposite direction, this maintains large concentration gradient between water and blood.

122
Q

What is the concentration of oxyge like in the water outside of the fish?

A

higher than in the blood, so as much oxygen as possible diffuses from the water into the blood.