Genetic Tech Flashcards
What is ELISA?
Protein based tests that uses a solid surface which is coated with antigen of interest.
If primary antibody binds to secondary, a colored product should appear
What can ELISA test for?
HIV
What is FISH/Karyotype analysis?
Used to locate specific DNA sequences on chromosomes in a person’s genome
Uses fluorescent probes which bind to chromosomal sequences
When can FISH be used?
To determine pre- and post-natal abnormalities
What is Hybrid capture assay (HCA)?
It is a nucleic acid hybridization targeting a DNA with an RNA probe
DNA:RNA hybrid that has chemiluminescent substrate added
What can HCA be used for?
HPV
What is DNA Microarray?
Extraction of mRNA from healthy person, cDNA is made fluorescent labded to identify affected genes
What is Microarray used for?
Breast cancer
What is restriction mapping?
Process of identifying the position of restriction
sites on a DNA fragment
What is restriction mapping used for?
Paternal testing
What is Sanger sequencing?
Extrude a DNA sequence to produce a single strand template, sequences done on gel
What is Sanger sequencing used for?
Breast cancer
What is Western blott?
Used to identify specific proteins from a complex mixture of proteins
when extracted from cells
What is western blott used for?
Creutzfeldt-Jakob Disease (CJD)
