Toxins

Question 1

What is the Enzymatic activity of Cholera Toxin?

Answer 1

ADP ribolsylates eucaryotic adenylate cyclase Gs

Question 2

What is biological effects of Cholera Toxin?

Answer 2

Activates adenylate cyclase. Increases level of cAMP which promotes secretion of fluid in intestinal epithelium leading to diarrhea

Question 3

What is enzymatic activity of Diphtheria toxin?

Answer 3

ADP ribolyates elongation factor 2

Question 4

What is Biological effects of Diphtheria toxin?

Answer 4

Inhibits protein synthesis in animal cells resulting in death of the cell

Question 5

What is enzymatic activity of Pertussis toxin?

Answer 5

ADP ribosylates adenylate cyclase Gi regulatory protein

Question 6

What is Biological effects of Pertussis toxin?

Answer 6

Blocks inhibition of adenylate cyclase, increasing levels of cAMP affects hormone activity and reduces phagocytic activity

Question 7

What is the Enzymatic activity of E. Coli Heat Labile Toxin (LT)?

Answer 7

ADP ribosylates adenylate cyclase GS regulatory protein

Question 8

What is enzymatic activity of Shiga toxin?

Answer 8

Glycosidase cleavage of rRNA

Question 9

What is Biological effects of Shiga toxin?

Answer 9

inactivates the 60s ribosomal protein and leads to inhibition of protein synthesis. Leads to Diarrhea, hemorrhagic colitis and Hemolytic uremic syndrome (HUS)

Question 10

What is enzymatic activity of Pseudomonas Exotoxin A?

Answer 10

ADP ribosylates elongation factor 2 (just like diphtheria toxin)

Question 11

What is the biological effects of Pseudomonas Exotoxin A?

Answer 11

Inhibits protein synthesis in susceptible cells, resulting in death of the cell

Question 12

What is the enzymatic effects of Botulinum toxin?

Answer 12

Zn++ dependent protease acts on synaptobreven at motor neuron ganglioside

Question 13

What is biological effects of Botulinum toxin?

Answer 13

Inhibits presympathetic Ach release from peripheral cholinergic neurons. Resulting in FLACCID paralysis

Question 14

What is the enzymatic effects of Tetanus toxin?

Answer 14

Zn++ dependent protease acts on synaptobrevin in CNS

Question 15

What is the biological effects of Tatanus toxin?

Answer 15

Inhibits NT release from inhibitory neurons in the CNS. Resulting in SPASTIC paralysis

Question 16

What is enzymatic effects of Staph Aureus Exfoliatin toxin B?

Answer 16

Cleaves desmoglein 1, and cadherin found in desmosomes in the epidermis (superantigen)

Question 17

What is the biological effects of Staph. Aureus Exfoliatin toxin B?

Answer 17

Seperation of the stratum granulosum of the epidermis. Between layers and the superficial dead space

Question 18

What is the bacterial source, target and disease of Perfringiolysin O toxin?

Answer 18

• Closteridium perfringes
• Cholesterol
• Gas gangrene

Question 19

What is the bacterial source, target and disease of Hemolysin?

Answer 19

• E. Coli
• Cell membrane
• UTI!!!!!

Question 20

What is the bacterial source, target and disease of Listeriolysin?

Answer 20

• Listeria monocytogenes
• Cholesterol
• systemic; meningitis

Question 21

What is the bacterial source, target and disease of Anthrax EF?

Answer 21

• Bacillus anthracis
• Cell membrane
• Anthrax (edema)

Question 22

What is the bacterial source, target and disease of Alpha toxin?

Answer 22

• Staph aureus
• cell membrane
• abcesses

Question 23

What is the bacterial source, target and disease of Pneumolysin?

Answer 23

• Strep. pneumoniae
• Cholesterol
• pneumonia; Otitis media

Question 24

What is the bacterial source, target and disease of Streptolysin O?

Answer 24

• Strep. pyogenes
• Cholesterol
• strep throat

Question 25

What is the bacterial source, target and disease of Leukocidin?

Answer 25

• Staph. aureus
• pahgocyte membrane
• pyogenic infections