Flashcards in Wagner Day 18 Deck (21)
S5, S6 and intervening loop do what?
collective interaction selective filter
Selective filter needs what two amino acids?
lysine at 1422, alanine 1712
ion pore is made up of
S5, S6 intervening loop
How many domains does Beta have?
in Na+ gate
how many domains does alpha have in Na+ gate
4 domains , 6 protiens in membrane for each domain
TTX inhibits which gate? and what amino acids are sensitive to it?
Na+ gate glutamate E387,tyrosine 385
Channel activation has S(4/5)
S4 alpha has which two amino asides
lysine and arginine
Inactivation determinants are from Domain (3/4/5)
Domain 3 and 4
Domain 3 and 4 have which amino acids?
is it for activation or inactivaitn
isoleucine, phenylalanine, methionine
for K gates what makes up the inactivation gate?
-N terminal or beta subunit with cytoplasmic loop that connects S4 and S5
(L/N/PandQ/T) highest rate of inactivation, lowest threshold
(L/N/PandQ/T) blocked by shellfish w conotoxins
(L/N/PandQ/T) blocked by spider toxins agatoxins
(L/N/PandQ/T) -blocked by dihydropyridine drugs
Ca2+ gate has how many subunits and is it covalently bonded?
5 , yes
Glutamate in the Ca2+ channels does what?
-provides high affinity Ca2+ binding
5HT2 -> Gq
5HT3 receptor is a ligand-gated ion channel that allows Na+ and Ca2+ influx, and K+ efflux.
5HT4-7 receptor systems are poorly understood.
M1, M3 & M5
M2 & M4