vacuole inheritance Flashcards Preview

Genetics of cell growth and division > vacuole inheritance > Flashcards

Flashcards in vacuole inheritance Deck (13)
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1
Q

vac8 binds vacuole and recruits what?

A

vac17 which recruits myo2

2
Q

what phosphrorylates vac17?

A

cdc28

3
Q

what mediates temporal regulation of vacuole transport`?

A

phosphorylation of vac17 and myo2

4
Q

vac17 is spatially regulated by being broken down where?

A

in bud

5
Q

what does dma1 recognise?

A

phosphorylated T240 in PEST sequence of vac17

6
Q

what does dma1 do to vac17?

A

ubiquitinates it to release it from myo2 and can be degraded by proteasome

7
Q

what must also phosphorylate vac17 S222?

A

cla4

8
Q

what recruits cla4?

A

cdc42

9
Q

what is s222 phosphorylation required for?

A

Dma1 dependent vac17 phosphorylation

10
Q

what inhibits kin4 in bud

A

cla4 which recruits lte1 that inhibits cla4

11
Q

what inactivates the dma1 dependent breakdown pathway?

A

kin4 and frk1 in mother cell

12
Q

why are kin4 and frk1 required for vacuole transport

A

they prevent breakdown of vac17 in mother

13
Q

what is FM4-64

A

a dye that is endocytosed and ends up in vacuoles