Unit 1 Section 1: Studying cells- Microscopes Flashcards Preview

AS Biology OCR > Unit 1 Section 1: Studying cells- Microscopes > Flashcards

Flashcards in Unit 1 Section 1: Studying cells- Microscopes Deck (11)
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1
Q

Define magnification

A

How much bigger the image is than the sample(specimen)

2
Q

Define Resolution

A

How detailed the image is, it is the ability of a microscope to distinguish between to points that are close together

3
Q

sum for magnification

A

magnification = length of image / length of specimen

4
Q

two types of microscope

A

Electron

Light

5
Q

two types of electron microscope

A

Scanning (SEM)

Transmission (TEM)

6
Q

Advantages/Disadvantages of TEM

A

Advatages- Provide high resolution images

Disadvantages- Specimen need to be sliced thinly.

7
Q

Advantages/Disadvantages of SEM

A

Advantages - Show the surface of the specimen and can be in 3D

Disadvantages - Lower Resolution than TEM

8
Q

Max Resolutions

A

Light microscope - 0.2um
TEM - 0.0001um
SEM- 0.005um

lower resolution the better

9
Q

Max Magnification

A

Light microscope - x1500
TEM - more than x1,000,000
SEM- less than x1,000,000

higher magnification the better

10
Q

dye examples

A

methylene blue- stains RNA/DNA

eosin - highlights cell membrane

11
Q

Electron micrograph images

A

are always black and white (but sometimes colour is added artificially to images don’t confuse this with staining)