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Flashcards in Translation Deck (10)
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1
Q

What is the role of Dicer and RISC?

A

Silencing of translation

2
Q

What are the steps used in mammalian RNAi?

A
  1. Processing dsRNA NFP 21-23 Nt fragments

2. Antisense strand of siRNA guides cleavage

3
Q

How are nucleotide sequences written?

A

5’ - 3’

4
Q

What are the three stop codons?

A

UAA,UAG,UGA

5
Q

What amino acids are paired with which homopolymer?

A

UUU - Phe

AAA - Lysine

CCC - Proline

6
Q

Describe the structure of tRNA

A

Amino acid binding site (Acceptor stem)

  • Aminoacyl-tRNA Synthetase reads and adds appropriate AA to stem
  • VERY SPECIFIC

Anticodon Loop:
- Complementray sequence to codon which binds to mRNA codon

7
Q

Differentiate IF2 and IF3

A

IF3 Binds to small subunit and keeps it apart from large subunit

IF2 binds to initiator tRNA and brings it to P site of small subunit
* Initiator tRNA contains methionine

8
Q

What is the function of the Kozak sequence?

A

Facilitates the ID of the start codon

9
Q

How do antibiotics such as streptomycin work?

A

Inhibition of protein synthesis

10
Q

Describe the pathology of Diphteria?

A
  • Produces toxin which InActivates elongations factor EF2

- Infection —> Tough leathery Membrane in pharynx