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Flashcards in stains Deck (19)
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0
Q

Basic stain

A

Cationic-chromogen exhibits poss charge
Strong affinity to neg charge cell components
More commonly used for bacterial satining.

1
Q

Acidic stain

A

Anionic-chromogen portion has neg. charge

Strong affinity for positive components of cell

2
Q

Acid fast

A

Stain used for pathogenic bacteria for humans such as M.tuberculosis and M.leprae.

3
Q

Acid fast stain

A

Primary stain- carbol Fuchsim
Decolorizing- acid alcohol
Counter stain- methylene blue

4
Q

Color of acid fast

A

P-Carbol Fuchsim- red
D-Acid-alcohol- red
C-Methylene blue- red

5
Q

Color of Non acid fast

A

P-Carbol Fuchsim-Red
D-acid alcohol- colorless
C-Methylene blue-blue

6
Q

Endospore stain

A

Primary stain-Malachite green
Decolorizing agent- water
Counter stain- safranin

7
Q

Endospore Primary stain

A

Malachite green- flood smear for 2-3 minutes over warm hot plate

8
Q

Decolorizing agent

A

Water- removes excess malachite green.

once spore accepts green, it can’t be decolorized by water.

9
Q

Counterstain

A

Safranin-red stain used as second reagent.

Colors decolorized vegetative cells, appear read after absorption of counter stain

10
Q

Spore stain- color of stain

A

P-malachite green-Green
D-water- Green
C-Safranin-Green

11
Q

Spore stain-Color of vegetative cell

A

P-Malachite green-Green
D-Water-Colorless
C-Safranin- Red

12
Q

Spore staining procedure

A

1) flood smear with malachite and place on top of warm water beaker for 2-3 min. Don’t let it evaporate
2) remove slide from beaker, cool, and wash with tap water.
3) Counterstain with safranin for 30 sec.
4) Wash with tap water
5) dry slide with bibulous paper.

13
Q

Gram stain

A

Primary stain-crystal violet
Mordant-Iodine
Decolorizing agent- alcohol-acetone
Counter stain-safranin

14
Q

Gram Stain procedure

A

1) gently flood smear with crystal violet for 1 min
2) gently wash with tap water
3) gently flood smear with Iodine mordant for 1 min.
4) gently wash-tap water
5) decolorized with alcohol-acetone
6) wash with tw
7) Counterstain safranin for 45 sec.
8) wash with tw
9) dry with bibulous paper and examine oil immersion

15
Q

Color of G+ cells

A

P-Crystal violet- purple
M-Iodine- purple
D- alcohol-acetone- purple
C- Safranin- purple

16
Q

Color of G- cells

A

P-crystal violet- purple
M-iodine- purple
D-alcohol acetone- colorless
C-Safranin- red

17
Q

Simple staining

A

Single reagent smear. Produces distinct contrast between organism an it’s background.

18
Q

Simple staining procedure

A
1) flood smear with indicated stain
 Carbol Fuchsim 15-30 sec
Crystal violet 20-60 sec
Methalyne blue- 1-2 min
2) wash smear with tw
3) dry using bibulous paper
4) repeat procedure for 2 rem. stains
5) oil immersion