Flashcards in Soya Deck (24)
What is the difference between soya consumption in americans and japanese?
americans eat about 3 pounds soy food/year whereas japanese ear about 24 pounds per year
What link is there between breast and prostate cancer and soya ?
japanese women who eat a lot more soya food compared to americans have had the rate of breast cancer in comparison
death rates from prostate cancer is lower in asian countries in comparison to americans
when asians migrate to us breast cancer and prostate cancer risks increases
What was the assumption with soya?
thought it may protect against hormone dependent cancer
- countries that ate lots of soya seemed to have a lot less hormone dependent cancers
What compound in soya is related to estradiol and what does estradiol do which has been related to the effects of soya?
equol is the related compound in soya
- both estradiol and equol have phenolic components and hydroxyl components that are almost exact same distance apart
estradiol is involved in cell division and has carcinogenic effects so therefore this is thought to be associated to the mechanism of hormone related cancers
What is equol ?
a metabolite (from the gut microflora) of daidzein, an isoflavone in soya
- you dont find equol in soya
What is a major source of phytoestrogens ?
major source is soya - isoflavones: geinstein and daidzrin- levels vary from 1-100mg/100g
What are the levels of phytoestrogens in soya milk and different soya protein fractions?
they are reduced compared to natural soya because some of the isoflavones are washed away
What happens to fermented soya?
levels of phytoestrogens are reduced (and proportions of glycosides to aglycones change)
Where are lignans founds ?
present in tea, cereals, berries, linseed oil, oat meal, lentils and asparagus- levels vary in these sources
most commonly found in western diets
they are in vivo metabolites
Where are sources of isoflavones found?
soya flakes, tofu, soya flour and soya sauce, levels vary from 566-23 mg/g
What compounds are all found in soya and all have a close structural relationship ?
daldzein, geinstein, coumestrol and equol and biochanin A
Why are synthetic oestrogen's bad?
they are known to be carcinogenic eg. hexestrol
zearalenonne causes reproductive disorders in animals
How is equol produced and what is interesting about it ?
produced by gut microflora
you get poor and good equol producers- the good ones are able to produce at least 100x more equol - therefore if equol has substantial biological activity then you would expect to see very varying effects between individuals - nothing has yet been found
What do hormones do ?
regulate almost every biological process:
- growth and development
- mental development, mood and memory
- sexual maturation
- immune function
What is the oestrogen receptor and what is it involved in ?
it belongs to the nuclear receptor super family of ligand regulated transcription factors
controls cell growth and differentiation in a diverse array of tissues:
- reproductive system
- cardiovascular system
- central nervous system
What happens when oestrogen binds to its receptor?
estrogen binds to inactive complex in the nucleus and activates it - causes ligand binding dimerisation DNA binding
dimer goes off to bind to DNA- binds to specific transcription factors and alters specific genes to produce enzymes and proteins
if the molecule binds to the inactive complex and it remains inactive then its said to be behaving as an antagonist preventing natural compounds binding
What do the levels of estrogenicity of phytoestrogens vary between in comparison to estradiol?
vary from 0.20 to
What links have been made between phytoestrogens and cancer prevention ?
hormone-related cancers are higher in western populations compared to certain asian populations
epidemiology links the consumption of soya and soya-derived foods as protective
mechanistic evidence suggests that certain phytoestrogens could play a role in prevention of hormone-related cancers
What are the differences in the levels of genistein and daidzein in blood between Finnish and Japanese men (ng/ml) ?
Finnish men- genistein (1.7) and daidzein (1.6) whereas in japanase men- genistein (74.6) and daidzein (27.2)
these levels could be very significant biologically
What discovery was observed about conjugated isoflavones and why was it relevant ?
45mg per day for 1 month led to an increase in follicular phase length and delayed menstruation
- fed the levels you expect in soya and in conjugated form
- if the menstrual cycle was a day longer and you multiplied this by a year, 10 years, 50 years, you'd be getting quite a substantially reduced amount of oestrogen stimulation
- therefore could this reduce cell division and was this the protective effect of soya
- the phytoestrogens would be acting to block the endogenous actions of estradiol
if this was happening across a population then you'd expect to see a reduction in fertility
NO actual evidence for reduced fertility due to increased soya consumption
There was a suggestion that the phytoestrogens were acting as agonists in post-menopausal women and antagonists in pre-menopausal women but how ?
there are 2 oestrogen receptors so maybe this explains the different effects
alpha and beta receptors
they are similar in many ways - DNA binding domain has 90% homology but ligand binding domain only have 10% homology
also have differences in tissue distribution and promoter specificity
although this demonstrates that phytoestrogens could act different in pre and post-menopausal women why does adding oestrogen activity after the menopause induce a beneficial effect
Overall what are the results for isoflavones and lignans?
epidemiology is less good for isoflavones and not good for lignans
the understanding of these compounds is very much lacking
What was observed when whole soy extract and soy isoflavones were compared ?
they looked at the activity of these compounds on apoptosis in prostate cancer cells
- the extract worked the best therefore it is not the structure of estradiol thats involved in the mechanism