Small group 1 Flashcards

1
Q

How can histology be defined?

A

Histology is the study of microscopic structures

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the steps to tissue preparation?

A
  1. Fixation
  2. dehydration
  3. Embedding
  4. sectioning
  5. staining
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is fixation and why is it done?

A

This is the process that preserves histological sections

The goal is to preserve tissues as closely to the natural state as possible.

why?

  1. maintain cell and tissue morphology
  2. prevent decay
  3. terminates biochemical reactions
  4. crosslinks proteins
  5. kills microorganisms
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

How would a tissue be fixated for a light microscope?

A

formaldehyde or formalin (37% aqueous solution of Fa)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

how would a tissue be fixed for an electron microscope?

A

glutaraldehyde (primary)

osmium tetroxide (secondary) for lipids!

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How are tissues dehydrated?

A

A series of alcohol solutions of concentrations beginning at 10% and gradually increasing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How would embedding be done if using a light microscope?

A

paraffin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

How would embedding be done if using an electron microscope?

A

epoxy resin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How should a tissue be sectioned for a light microscope?

A

5 - 15 micrometers thick

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How should a tissue be sectioned for an EM

A

0.05 - 0.09 micrometers thick

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Explain the hematoxylin and Eosin stain?

A

hematoxylin is a basic positive dye that normally reacts with the nucleus
The stain is blue in color

Eosin is an acidic dye that has a net negative charge that normally interacts with the cytoplasm.
The stain is pink in color

does not stain lipids well

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Explain Mallory’s trichrome stain

A

aniline blue, acid fuchsin, and orange G

stains connective tissue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Masson’s Trichrome stain

A

smooth muscle will be stained red and connective tissue will be stained blue/green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What dyes stain lipids well?

A

oil red O

osmium tetroxide

sudan black

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Explain what the silver stain does?

A

will stain reticular fibers black

H&E will not stain fibers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

periodic acid schiff

A

Is used to stain carbs and carb rich molecules

color pink/magenta

17
Q

What stains will stain elastic fibers?

A

weigerts

orcein

verhoeffs

18
Q

explain IHC?

A

antibody-antigen interactions for labeling cellular components

antibodies can be monoclonal or polyclonal

19
Q

Direct vs indirect immunoflurescence?

A

Direct- antibody to antigen

Indirect- primary antibody and color antibody

20
Q

TEM vs SEM vs LM

A

TEM is used to examine the internal structure of an anatomical part

SEM is used to examine the surface of a tissue

LM is used to examine the surface of an tissue ( no electrons)