Session 1: Investigative Techniques Flashcards

1
Q

What is used to preserve samples in light microscopy?

A

Formalin

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2
Q

What is used to embed samples for light microscopy?

A

Melted paraffin (allows sample to be sliced thinly as it sets hard once cooled)

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3
Q

What does haematoxylin stain?

A

Stains the nucleus blue.

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4
Q

What does eosin stain?

A

Cytoplasm and ECM pink

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5
Q

TRUE OR FALSE: high frequency waves have a longer wave length than low frequency waves.

A

False

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6
Q

What does TEM stand for?

A

Transmission electron microscopy

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7
Q

What is a disadvantage of TEM?

A

Uses an electron beam generated in a vacuum therefore cannot be used for living samples.

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8
Q

What is a sample fixed with during TEM?

A

Glutaraldehyde

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9
Q

What is a sample embed in during TEM?

A

Epoxy resin

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