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Flashcards in Sequencing Quick Info Deck (7)
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1
Q

3 Proteases used for larger sequencing

A

Trypsin
Chymotrypsin
Carboxypeptidase

2
Q

Edman degradation

A

removes the N-terminal amino acid

If you have 2 n-terminal amino acids, probably have a disulfide bond

3
Q

Trypsin

A

a protease that cuts after positively charged amino acids

4
Q

Chymotrypsin

A

a protease that cuts after an aromatic group

5
Q

Carboxypeptidase

A

cuts away the C-terminal amino acid

6
Q

Reducing agents for disulfide bonds

A

B-mercaptoethanol (B-ME) and dithiothreitol (DTT)

7
Q

Cyanobromide

A

a reagant (way to break a protein without a protease)

breaks a sequence after a methionine