Schultz-Oct 9 Flashcards Preview

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Flashcards in Schultz-Oct 9 Deck (13)
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1
Q

chromatin

A

DNA + histones + any other proteins associated with DNA

2
Q

nucleosome

A

histone core + DNA wrapped around in histone core

3
Q

nucleosome core particle

A

H2A, HB, H3, H4

4
Q

how many copies of H2A/H2B dimers in nucleosome core?

A

2

5
Q

How many copies of H3/H4 tetramer in nucleosome core?

A

1

6
Q

3 histone chaperones

A

CAF1, Asf1, and NAP1

7
Q

Where does CAF1 and Asf1 bind on histone?

A

H3-H4

8
Q

Where does NAP1 bind n histone?

A

H2A-H2B

9
Q

Function of histone chaperones

A

promote initial formation of histone-DNA complex

10
Q

3 DNA-dependent ATPases

A

ACF, RSF, Chd1

11
Q

Which 2 DNA-dependent ATPases need chaperones?

A

ACF and RSF

12
Q

Which chaperone shield histone surfaces that interact with DNA?

A

Nap1

13
Q

What do you get on gel when you do not use ATP in chromatin assembly?

A
  • monosomes, but no disomes or higher order ones