RNA processing Flashcards Preview

BMS I > RNA processing > Flashcards

Flashcards in RNA processing Deck (7)
Loading flashcards...
1
Q

5’ splice site

A

GU-AAGU (or something similar)-GU is important

2
Q

Branch site

A

Catalytic A close to 3’ splice site

3
Q

3’ splice site

A

AG requirement then conserved run of U or C

4
Q

splicing mechanism

A

3’ AG, phosphate bond to catalytic A 2’OH, 3’AG to the phosphate is cleaved making into 3’OH, phosphate bond to phosphate by 5’ G-slide 7
2’OH is a nucleophile that breaks phosphate bond
The bonds form/break are called transesterification
DRAW

5
Q

SNURPS+parts of it

A

wraps sequence in a knot

U1/U2-bring 5’ and 3’ splice site in 3D proximity

6
Q

Alternative spliciing

A

Idea of being able to keep some exons and not others to make differing proteins
Result in many different related proteins form a single gene

7
Q

Beta thallasemia

A

Mutation in intron, splicing occurs more upstream-more amino acids in gene