Microscopy part 2 Flashcards Preview

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Flashcards in Microscopy part 2 Deck (14)
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1
Q

what is the stain used in acid fast staining?

A

fuschin

2
Q

How does acid fast staining work?

A

fuschin is added into mycobacteria under high high heat, the solution cools down, mycobacteria harden, and then everything is washed with acid alcohol, removes stain everywhere except the mycobacteria

3
Q

what two gram positive groups make spores

A

bacillus and clostridium

4
Q

how does spore staining work?

A

high heat makes them permeable, malachite green stains everything. the mixture cools, everything washed, then stained with safranin. green still stay in spores, but everything else is red

5
Q

define sporangium

A

bacterial cell with endospore

6
Q

define vegetative cell

A

bacterial cell that is just chillin

7
Q

define endospore

A

spore that is still within the sporangium

8
Q

how does vital staining work?

A

Sytox green (SG) binds to DNA of dead cells, not live ones, because the DNA is easy to get at since the cell membrane is damaged. DAPI blue is then used, which can stain the DNA of everything. Therefore, live = blue, dead = purple

9
Q

how does metabolic staining work?

A

CTC is used, which gets gets reduced by succinate dehydrogenase to a red fluorescent colour. therefore, red cell are undergoing metabolism.

10
Q

what is the stain used to stain dead DNA?

A

sytox green

11
Q

what is the stain used to stain all DNA, live and dead?

A

DAPI blue

12
Q

what is the stain used in metabolic staining?

A

CTC red

13
Q

what is the secondary antibody that binds to the primary antibody in immunofluorescence

A

fluorochrome

14
Q

what do fluorochromes emit?

A

visible light when excited by UV