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Introduction to Research and Experimental Skills > Metabolomics - NMR > Flashcards

Metabolomics - NMR Flashcards

1
Q

What is metabolism?

A

The integration of physical and chemical processes employing small biochemicals involved in

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2
Q

What is metabolomics?

A

The global analysis of metabolites, small molecules generated in the process of metabolism.

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3
Q

What is metabonomics?

A

Studying the response to drugs of metabolites

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4
Q

What are the metabolomic technologies?

A

NMR Spectroscopy

Mass spectrometry

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5
Q

What are the advantages of NMR?

A 
Solution states
MAS (tissue extracts, tissue)
Invivo spectroscopy
relatively robust
Short time taken
Non-destructive analysis
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6
Q

What are the advantages of Mass spectrometry?

A

highly sensitive
can analyse multiple samples at one time
Has a good ability to identify metabolites.

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7
Q

What are the requirements for perfect metabolomic method?

A

sensitivity
reproductability
…

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8
Q

What are the disadvantages to NMR?

A

Sensitivity is relatively poor

Compound identification may have samples overlapping.

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9
Q

What does NMR observe?

A

Based off the properly of the nucleus of some atoms termed the spin.
Spins behave as if they have positively charged particles rotating in a magnetic field
Rotation in the field produces a magnetic moment.
Two possible orientations of rotation lead to two energy states related to alignment with or against the outside magnetic field
most NMR applications use spin-1/2 nuclei with exactly two energy states

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10
Q

What are some isotopes used in NMR?

A

Hydrogen
N15 (has to be enriched in molecules)
C13

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11
Q

How does NMR work?

A

Between the two states there is an energy difference which is proportional to the magnetic field.

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12
Q

What can NMR distinguish?

A

Spin-1/2 atom in molecules based on their resonance frequency

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13
Q

What is the chemical shift in NMR?

A

Spin-1/2 atom in molecules based on their resonance frequency - the chemical shift.

The chemical shift changes as a consequence of the surrounding electron density which is directly related to the chemical bonds.
the chemical shift is therefore highly sensitive to ….

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14
Q

What is the signal intensity proportional to?

A

The number of spins

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15
Q

What is univariate data?

A

Test hypothesis for individual variables

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16
Q

What is multivariate data?

A

Test hypothesis for multiple variables (metabolites and the response to one changing)

17
Q

What is STOCSY?

A

Statistical correlation spectroscopy

Used to detect correlated and anti-correlated changes

18
Q

What is the main clinical test that use NMR?

A

Tests for cholesterol

MRI

Introduction to Research and Experimental Skills (7 decks)

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