MCM 2-28 Microanatomy of Connective Tissue Flashcards Preview

MSI Unit II > MCM 2-28 Microanatomy of Connective Tissue > Flashcards

Flashcards in MCM 2-28 Microanatomy of Connective Tissue Deck (50)
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1

definition of connective tissue

functions?


examples?

tissue that forms a continuum with the other 3 major tissues to maintain a functionally integrated body

mostly structural. characterized on basis of its ECM

organ capsules, tendons/ligaments, areolar tissue filling spaces, fat, cartilage, bone.

2

connective tissue develops from

embyronic mesenchyme, which forms from embryonic neural crest

embyronic neural crest -> embryonic mesenchyme -> connect tissue

3

describe mesenchymal appearance

what can they function as in adult tissue?

oval nucleus, prominent nucleoli, small amount of cytoplasm

stem cells

4

if a cell has lots of euchromatin

it is replicatively/synthetically active

5

connective tissue has two components

ECM - ground substance, protein fibers, tissue fluid (blood/plasma)

and connective tissue cells (resident and immigrant) CT cells

6

resident CT cells

arise in CT, spend entire life there

create the fibers and ground substance of CT, along with adipocytes

mesenchymal cells, fibroblasts, fibrocytes, reticular cells, adipocytes

7

immigrant CT cells

arise in hematopoetic bone marrow stem cells, migrate into the CT

macrophages, mast cells, plasma cells, leukocytes, eosiniphils (lots of cells from bone marrow)

8

-cyte?

blast?

cyte is a more mature form

blast is a cell that prolfierates and gives rise to other cells

9

fibroblast appearance

euchromatic nucleus, lightly stained

lots of organelles in cytoplasm

10

fibrocyte role and appearance

focused on secretion of collagen and ground substance. no longer proliferating

nucleus is more condensed heterochromatin, not the active euchromatin-like fibroblast.

11

blasts vs cytes

blasts - highly euchromatic nucelus, packed with organelles (golgi, ER) and irregular shaped

cytes - spindle shaped, heterchromatic necleus, fewer organelles, embedded within matrix.

12

reticular cell role and appearance

fibroblast like cells that produce reticular fibers in hematopoietic, lymphoid, and adipose tissue

stellate shaped cells with oval euchromatic nucleus that has prominent nucleolus

13

adipocytes

single lipid dropet that pushes cytoplasm and nucleus to the periohery (signet ring cell).

brown (multilobular) and white (unilobular)

14

describe macrophages

apperance?

how do we ussualy idenfity?

monocytes travel in blood and migrate into CT to form macrophages

smaller than fibroblast, heterochromatic KIDNEY SHAPED nucleus and cytoplasm filled with granules. Dark heterochromatin ring around periphery. lots of lysosomal vacoules

idenfity in animals by injecting trypan blue (colloid dye)

15

Mast cells

largest of CT cells

cytoplasm filled with membrane-bounded basophilic granules that contain heparin, chondriton sulfate, and ECF-A

allergic reactions, found around blood vessels

light colored cytoplasm with darker central nuclues

16

plasma cells

differentiate from antigen stimulated B-cell

large, ovoid cells

eccentric nucleus, abundant RER, "CLOCK FACE" nucleus, clear zone near nucleus contains golig and centrioles

17

ground substance description

consists of?


functions as?

viscous mixture that binds cells to CT fibers

consists of GAGS, proteoglycans, and multiadhesive glycoproteins (lamin and fibronectin)

functions are both structural and physiological - homrones and growth factors have resovoirs in GS

18

CT fiber types

collagen - >50nm fibrils, 1-20 micron fibers. Thickest

Reticular fibers -

19

describe collagen type 1 (exam q)

forms triple helix, when bind to one another, form 64 periodicity. striped. form tendon

most widespread, most abundant protein in the body (90%). resists tension like in tendons

20

collagen 3

forms reticular fibers, flexible meshwork

21

collagen 2

cartilage, resists pressure

22

collagen 4

important component of basal lamina

23

collagen 7

anchores basement membrane to collagen fibers

24

basal lamina vs basement membranes

BL - lamina lucida and lamina densa

bm = basal lamina + reticular fibers

25

Osteogensis Imperfecta

single nucleotide change in collagen I gene
produces aortic rupture, fractures

26

Scurvy (vitamin C deficiency)

impaired wound healing, issues with collagen synthesis due to lack of vitamin c

27

Ehlers-Danlos type IV

type III collagen problem
aortic/intestinal ruptures

28

Marfan syndrome

mutant fibrillin, elastin core can overstretch, danger of blood vessel rupture

29

CT fiber staining


collagen -
reticular -
elastic -

collagen - pink in H&E

reticular - pink in H&E, indistinguihsable form collagen without silver stain

elastic - pink in h&e, not distinguishable from collagen without resorcin-fuschin or picro-orcelin

30

Loose areolar CT

found where?

characteristics?

located where?

found in intervening spaces

more cellular than dense CT
lots of resident cells
found in lamina propria, mesentary, and pappilary layer of dermis

positioned between tissues to allow movement

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