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Flashcards in Match the tube to the test Deck (15)
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1
Q

Tubes 3 & 4 Reagent?

A

Urea Hydrolysis/ Urease test Urease and Phenol Red 3(+), 4(-)

2
Q

Tubes 1 & 2 Reagent?

A

ONPG test onpg disk 1(+), 2(-)

3
Q

Plate 7 & 8 Reagent?

A

Starch Hydrolysis test Iodine 7(+), 8(-)

4
Q

Tubes 5 &6 Reagent?

A

Phenylalanine Deaminase test Ferric Chloride 5(+), 6(-)

5
Q

Tubes 9 & 10 Reagent?

A

MR test Methyl Red 9(+), 10(-)

6
Q

Tubes 19 & 20 Reagent?

A

Tryptophan Hydrolysis/ Indole Production test Kovac’s reagent 19(+), 20(-)

7
Q

Tubes 17 & 18 Reagent?

A

Simmon’s Citrate Bromthymol Blue 17(+), 18(-)

8
Q

Tubes 11 & 12 Reagent?

A

VP Test Alpha-napthol and KOH 11(+), 12(-)

9
Q

Tubes 15 & 16 Reagent?

A

Decarboxylation Test Bromocarbol Purple 15(+), 16(-)

10
Q

Tubes 21, 22, 23, 24

A

Kliger’s Iron Agar (KIA) 21 (Glu+, Lac+, Gas+, H2S-) 22 (Glu+, Lac-, Gas-, H2S+) 23 (Glu-, Lac-, Gas-, H2S-) 24 (Glu+, Lac-, Gas-, H2S-)

11
Q

Tubes 29, 30, 31 Determine positive?

A

Nitrate Reduction Test 3 Steps: Observe bubbles, reagent: Sulfanilic acid and alpha naphthylamine, Zinc 29 Bubbles+ 30 No Bubbles, Reagent turned red + 31 No bubbles, Reagent (no change), Zinc Turned rose-

12
Q

Tubes 25, 26, 27, 28

A

O-F test 25(+oxid, +glu aerobic) 26(+Ferm, +glu anaerobic) 27(+oxid aerobic) 28(-Ferm)

13
Q

Tubes 32, 33, 34

A

Sugar Fermentation test 32 (Bubbles+, Acid+) 33 (Bubbles-, Acid+) 34 (Bubbles-, Alkaline reaction)

14
Q

Catalase Test?

A

Identifies organisms that produce the enzyme catalase. Reagent: Hydrogen Peroxide Positive result= bubbles

15
Q

Oxidase test?

A

Identifies bacteria containing the respiratory enzyme cytochrome oxidase Reagent= oxidase