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Flashcards in Lecture 2 Deck (26)
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1

How many lens does a simple microscope have?

1

2

objective lens x ocular lens =

total magnification

3

the ability of the lens to distinguish 2 points

resolution

4

what is the resolution of the best light microscope

0.2 micron

5

Shorter wavelengths of light provide

greater resolution

6

measure of the light bending ability of a medium

refractive index

7

What is used to keep light from bending?

immersion oil

8

What kind of background are dark objects visible against

bright ones

9

What backgrounds are light objects visible against?

dark ones

10

Accentuates diffraction of the light that passes through a specimen

phase-contrast microscopy

11

Accentuates diffraction of the light that passes through a specimen; uses two beams of light

differential interference contrast microscopy

12

Absorb UV light and emit visible light

Fluorescence microscopy

13

popularly used as a tag to illuminate living cells in a florescent microscope

GFP

14

Uses electrons instead of light

Electron microscopy

15

Produces a three-dimensional image

Confocal microscopy

16

Specimens may be stained with heavy metal sales

Transmission electron microscopy (TEM0

17

Ultrathin sections of specimen. The light passes through the specimen, then an electromagnetic lens to a screen or film

TEM

18

Produces a beam of electrons that scans the surface of a whole specimen.

Scanning electron microscopy (SEM)

19

Secondary electrons emitted from the specimen produce the image

SEM

20

Uses a metal and diamond probe inserted into the specimen and produces three dimensional image

Scanned probe- Atomic force microscopy

21

Coloring the microbe with a dye that emphasizes certain structures

staining

22

A thin film of solution of microbes on a slide

smear

23

the chromophore is a cation

in a basic dye

24

the chromophore is an anion

An acidic dye

25

staining the background instead of cells

negative staining

26

use of simple basic dye

simple stain