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Basic Immunology- Module 1 > Laboratory diagnosis of infectious diseases > Flashcards

Flashcards in Laboratory diagnosis of infectious diseases Deck (16)
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1

Give examples of endo-parasites?

protozoa and helminths

2

What are the methods of lab diagnosis of infecitous disease?

microscopy; culture-based; serological; molecular and rapid point of care testing

3

What is group 1 hazard group?

unlikley to cause human disease

4

What is group 3 hzard group?

can cause severe human disease; may be a serious harzard to employees, may spread to the community but there is usually effective prophylaxis or treatment available

5

What 2 dyes are found in the Romanowsky stain?

cationic dye-azure-bind acid nuclei giving blue/purple; anionic-eosin which binds cytoplasm giving red

6

What is the india-ink stain used for?

cryptococcus neoformans

7

What is the gomori-grocott stain used for?

aspergillus fumigatus

8

What is chocolate agar?

heated blood to releases haem

9

What is the conventional method of bacterial identification?

culture; gram stain; catalase and agglutination; oxidase tests

10

What is the current method of bacterial identification?

mass spectometry: MALDI-TOF

11

What is AST?

antimicrobial susceptibility testing

12

What are the methods of AST?

disc diffusion and also minial inhibitory concentration

13

What technique is used for serology testing?

ELISA

14

Give an example of a point-of care testing method?

immunochromatography

15

How does immunochromatography work?

a sample is dripped onto a test strip which then moves by capillary action and the captured by a lablled antibody which then is captured by an anti-influenza antibody forming a line

16

Give an example of a molecular method of identification?

nucelic acid tests