Laboratory diagnosis of infectious diseases Flashcards Preview

Basic Immunology- Module 1 > Laboratory diagnosis of infectious diseases > Flashcards

Flashcards in Laboratory diagnosis of infectious diseases Deck (16)
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1
Q

Give examples of endo-parasites?

A

protozoa and helminths

2
Q

What are the methods of lab diagnosis of infecitous disease?

A

microscopy; culture-based; serological; molecular and rapid point of care testing

3
Q

What is group 1 hazard group?

A

unlikley to cause human disease

4
Q

What is group 3 hzard group?

A

can cause severe human disease; may be a serious harzard to employees, may spread to the community but there is usually effective prophylaxis or treatment available

5
Q

What 2 dyes are found in the Romanowsky stain?

A

cationic dye-azure-bind acid nuclei giving blue/purple; anionic-eosin which binds cytoplasm giving red

6
Q

What is the india-ink stain used for?

A

cryptococcus neoformans

7
Q

What is the gomori-grocott stain used for?

A

aspergillus fumigatus

8
Q

What is chocolate agar?

A

heated blood to releases haem

9
Q

What is the conventional method of bacterial identification?

A

culture; gram stain; catalase and agglutination; oxidase tests

10
Q

What is the current method of bacterial identification?

A

mass spectometry: MALDI-TOF

11
Q

What is AST?

A

antimicrobial susceptibility testing

12
Q

What are the methods of AST?

A

disc diffusion and also minial inhibitory concentration

13
Q

What technique is used for serology testing?

A

ELISA

14
Q

Give an example of a point-of care testing method?

A

immunochromatography

15
Q

How does immunochromatography work?

A

a sample is dripped onto a test strip which then moves by capillary action and the captured by a lablled antibody which then is captured by an anti-influenza antibody forming a line

16
Q

Give an example of a molecular method of identification?

A

nucelic acid tests