Investigative Techniques Flashcards Preview

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Flashcards in Investigative Techniques Deck (15)
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1
Q

What are the requirements to view a tissue under a light microscope?

A

Preserve, embed, stain

2
Q

What is used to preserve a tissue to be seen under a light microscope?

A

Formalin

3
Q

What is used to embed a tissue to be seen under a light microscope?

A

Melted Paraffin that sets hard when cooled

4
Q

What is used to stain a tissue to be seen under a light microscope?

A

Haematoxylin and Eosin

5
Q

What does Haematoxylin stain and what colour is it?

A

Stains the nucleus blue

6
Q

What does Eosin stain and what colour is it?

A

Stains the cytoplasm and extracellular matrix pink

7
Q

How is a frozen section done?

A
  1. Specimen is placed on a disc and frozen to -20 to -30 degrees and becomes rock hard
  2. Cryostat is used to cut the specimen
  3. Stain with Haematoxylin and Eosin
8
Q

What are the positives and negatives of using a Frozen section?

A

+-Takes less time

– less quality image

9
Q

How does a fluorescent microscope work?

A

Fluorophores are added to sample and emit light when irradiated by specific wavelengths of light and so shows the different components of a cell

10
Q

What do samples need to be like for a confocal microscope?

A

Thinly sliced otherwise light will not pass through

11
Q

What occurs in Autoradiography?

A

Molecules are labelled with radioactive markers which are injected into live tissues

12
Q

What are the specifications for a light microscope?

A

Magnification is 1000x

Resolution is 0.2 micrometres

13
Q

What are the specifications for a TEM?

A

Magnification is 250,000x

Resolution is 1nm

14
Q

What is the difference between NMR and MRI?

A

MRI is used in medicine

15
Q

What does a low frequency mean for light microscopy?

A

Less energy and poor resolution