Flashcards in Investigative Techniques Deck (15)
What are the requirements to view a tissue under a light microscope?
Preserve, embed, stain
What is used to preserve a tissue to be seen under a light microscope?
What is used to embed a tissue to be seen under a light microscope?
Melted Paraffin that sets hard when cooled
What is used to stain a tissue to be seen under a light microscope?
Haematoxylin and Eosin
What does Haematoxylin stain and what colour is it?
Stains the nucleus blue
What does Eosin stain and what colour is it?
Stains the cytoplasm and extracellular matrix pink
How is a frozen section done?
1. Specimen is placed on a disc and frozen to -20 to -30 degrees and becomes rock hard
2. Cryostat is used to cut the specimen
3. Stain with Haematoxylin and Eosin
What are the positives and negatives of using a Frozen section?
+-Takes less time
-- less quality image
How does a fluorescent microscope work?
Fluorophores are added to sample and emit light when irradiated by specific wavelengths of light and so shows the different components of a cell
What do samples need to be like for a confocal microscope?
Thinly sliced otherwise light will not pass through
What occurs in Autoradiography?
Molecules are labelled with radioactive markers which are injected into live tissues
What are the specifications for a light microscope?
Magnification is 1000x
Resolution is 0.2 micrometres
What are the specifications for a TEM?
Magnification is 250,000x
Resolution is 1nm
What is the difference between NMR and MRI?
MRI is used in medicine