Immunology in the Clinic and Research Lab Flashcards Preview

CLINICAL PATHOLOGY > Immunology in the Clinic and Research Lab > Flashcards

Flashcards in Immunology in the Clinic and Research Lab Deck (6)
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1

What is the antibody repertoire? 

Total possible number of antibody binding sites 

2

What is antibody affinity? 

Strength of a single interaction between an antibody and its epitope

3

What is antibody avidity? 

Sum of different affinities (Some antibodies will be multimeric and have several different binding sites) 

4

What is the half-life of antibodies? 

The amount of time an antibody will remain in the body 

5

Describe the polyclonal antibody response 

  • When we are infected with a pathogen we mount a polyclonal antibody response 
    • It is polyclonal because there are multiple epitopes on an antigen hence we produce a polyclonal response, antibodies with different binding specificities
  • B-cells in our bodies will have a unique specificity for an antigen, the binding of the epitope to the B-cell will induce B-cell proliferation and form a clone of B-cells 

6

How do we produce monoclonal antibodies? 

 

Monoclonal antibodies = Bind specifically to a single epitope 

  • Hybridoma culture 
    • We take the antigen we want to make the Ab's and inject it into the mouse 
    • After 2 weeks we will harvest the B-cells which make the antibody 
    • We take the B-cells and fuse them with myeloma cells (derived from B-cell tumour but do not produce antibodies themselves) 
      • These cells also lack the HGPRT gene (hypoxanthine guanine phosphoribosyl transferase)
    • After we have carried out the fusion process, we have a mixture of cells
      •  (unfused B-cells and myeloma cells and fused cells (called hybridomas)

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