HMP Flashcards Preview

DSNM > HMP > Flashcards

Flashcards in HMP Deck (11)
Loading flashcards...
1
Q

glucose 6P DH

A

uses NADP+ and H2O to make NADPH and 6-phosphogluconolactose

inhibited by NADPH

2
Q

Cell needs NADPH on HMP

A

@FA syn and detox

oxidative rxns produce NADPH
-nonoxidative rxns convert ribulose 5P to glucose 6P to produce more NADPH

use both pathways

3
Q

cell needs NADPH and pyruvate on HMP

A

@RBCs Oxidative and non oxidative rxns used

  • oxidative gen NADH ade ribulose 5P
  • nonox convert ribulose 5P to fructose 6P and glyceraldehyde 3P which glycolysis converts to pyruvate

use both pathways

4
Q

Pathways requiring NADPH

A

Reductive biosyn

cytochrome p450 monooxygenase system

phagocytosis by WBC

Syn of NO

Reduction of H2O2 (ROS)

5
Q

CYP450 activity

A
  1. Add OH group to make toxins/drugs more soluble
  2. Mitochondrial-biosyn of steroid hormones
  3. Mitochondrial-Liver-Biosyn of bile and active vit D3

Needs NADPH and Oxygen to function

6
Q

Glutathione reduction

A

Glutathione in reduced form=GSH

OHe- to H2O with glutathione

  • after reduces to H2O=becomes oxidized and inactive by glutathione reductase
  • NADPH re-reduces glutathione using glutathione reductase
7
Q

Superoxide reaction

A

Hb-Fe2+O2 to Hb-Fe3+ + O2e-
Spontaneous
Occurs in highly oxidizing environemtnof RBC
Why GSH is important

8
Q

Glucose 6P DH def what it does and then what occurs (4 things)

A

won’t make NADPH from glucose 6P (also can’t do HMP pathway)

  • ROS accumulate because no GSH re-reduction
  • Heinz bodies (precipitation of Hb) are symptomatic
  • Worsened by oxidant stress (infections/drugs/fava beans)
  • hemolysis by ROS
9
Q

Favism-Alternate name?

  • inheritance
  • homo vs hetero?
  • why occur?
  • Sx
A

Glucose 6P DH def
-x link recessive
hetero is usually asymptomatic if no oxidant stress
-offers relative resistance to type of malaria

Sx-Heinz bodies, anemia

10
Q

Malic enzyme

A

in Malate to pyruvate in lipid synthesizing cells (adipose, mammary gland, liver)

produces 70% total NADPH

11
Q

Nicotinamide Nucleotide Transhydrogenase

A

NADH to NADPH (and NAD+ obviously)

Close to glutathione reductase so can product vs ROS coming off ETC from CoQ