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Flashcards in Exam questions-Crawford Deck (16)
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1
Q

What innate response provides a first line of defense against bacterial infectiion

A

activation of the alternate complement pathway by the bacterial surfaces

2
Q

What is the most important diference between the classical and alternate complement pahtways

A

the classical pathway is activated by antigen:antibody complexes

3
Q

If an animal has a C3 deficiency, what would be the most common clinical sign

A

Recurrent bacterial infections

4
Q

What is the general purpose of immunodiagnostic assays

A

Detection of an antigen or antibody in patient samples

5
Q

What test will be performed on an animal suspected of rabies

A

direct immunoflourescent antibody test for rabies virus antigens in the brain tissue

6
Q

What test do you conduct to see if antibodies are in the blood

A

indirect immunoflourescent antibody test

7
Q

What is the correct combination of reagents to use in an ELISA to detect antibodies

A

EIA antigen to coat wells, patient serum, anti-(animal) IgG antibody conjugated to HRP as the detection antibody, HRP substrate

8
Q

What is the correct combination of reagents to use in an ELISA to detect antigne

A

anti-(disease) antibody to cat wells, patient serum, anti-(disease) antibody conjugated to HRP as the detection antibody, HRP substrate

9
Q

can you test for both an antigen and an antibody in the same test

A

no, the capture antibody will bind the capture antigen

10
Q

What would you add to a serum sample in order to capture heartworm antigen, FeLV antigen, and FIV antibody

A

mouse anti-heartworm antibody conjugated to HRP, mouse anti-FeLV antibody conjugated to HRP, and FIV antigen conjugated to HRP

11
Q

What complement path is activated by binding IgG to antigens on cell surface

A

Classical pathway

12
Q

What complement pathway is directly activated by bacteria in the absence of a humoral immune response

A

alternate pathway

13
Q

What is the key enzyme formed by both the classical and alternate complement pathways that allows for the completion of the cascade

A

C3 convertase

14
Q

What is the key protein generated by this enzyme that coats bacterial surfaces and infected cell surfaces

A

C3b

15
Q

What is the most important effector functions that result from complement activation by bacterial infection

A

lysis of bacteria, phagocytosis of bacteria, inflammation

16
Q

How would you test a blood sample for the presence of antigens from an infectious agent

A

direct imunofluorescent antibody test that uses the patient’s blood smear as a substrate and FITC-labeled antibody specific for agent