Escherichia coli - Exam 2 Flashcards Preview

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Flashcards in Escherichia coli - Exam 2 Deck (23)
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1
Q

E. coli is a gram ______ bacteria.

A

Negative

2
Q

True/False. E.Coli is part of the normal flora of animals.

A

True

3
Q

How is strain identification of E. coli commonly done?

A

Serotyping (ID of bacterial O:K:F:H antigens) and virotyping (ID by specific toxic activity, virulence factor or virulence gene)

4
Q

What are the antigens of E. coli?

A

O - LPS

K - capsule

H - flagella

F or P - adhesins

5
Q

What are the clincal signs for enterotoxigenic E. coli?

A

Diarrhea is water and non-bloody

Minimal inflammatory changes

6
Q

Which E. coli causes edema disease in weaned pigs?

A

Shigatoxigenic E. coli

7
Q

Which species/age group does enterotoxigenic E. coli affect?

A

Neonatal and weaned pigs

Neonatal calves and lambs

Dogs and horses

8
Q

How is E. coli typically transmitted?

A

Fecal-oral route

9
Q

What is the virulence factor for enteropathogenic E. coli?

A

Bundle forming pilus

10
Q

What protein is used for attachment by enterpathogenic E. coli? What is the effect of the attachment?

A

Initimin is an E. coli protein for attachment

Causes the collapse of microvilli in distal small intesting and upper large intestine

11
Q

What are the clinical sings of enteropathogenic E. coli?

A

Watery diarrhea in all animals

No blood or leukocytes

12
Q

Which type of E. coli is the causative agent for neonatal colibacillosis and colisepticemia in pigs?

A

Enterotoxigenic E. coli

13
Q

Which type of E. coli has zoonotic potential?

A

Enterohemorrhagic E. coli

14
Q

What does E. coli cause in dogs?

A

Colisepticemia in neonatal pups

Pyometra in bitches

Lower UTIs

15
Q

What does E. coli cause in poultry?

A

Omphalitis

Colisepticemia

Coligranuloma

16
Q

What is the disease caused by E. coli that is a peracute condition that results in death due to endotoxemia?

A

Coliform mastitis

17
Q

How do you diagnose enteric disease caused by E. coli?

A

Fecal sample for culture

18
Q

What test would you use to test for Lipid A?

A

Limulus Amebocyte (LAL) Test

Lipid A is the endotoxin of gram negative bacteria

19
Q

What is the benefit of having the family of enterobacteriaceae as part of the normal flora in the GI tract?

A

Microbial antagonism - protect GI tract against foreign bacteria

Breakdown and adsorption of the food

Waste processing

Vitamin K production

20
Q

What are the most prevalent bacteria in nosocomial infections?

A

Infections acquired from the hospital

Klebsiella pneumonia

Klebsiella oxytoca

Enterobacter cloacae

Proteus mirabilis

Citrobacter freundii

21
Q

Which enterotoxins of E. coli cause diarrhea?

A

AB toxins

They are heat liable and stable, both plasmid encoded

22
Q

What are the virulence factors of necrotoxigenic E. coli (NETC) and what does it cause?

A

Cytotoxic necrtizing factors 1 and 2 (cause necrosis of cells…obvii)

Causes UTI infections (or so they think, “they” aren’t really sure)

23
Q

Which rough strain is used to make a vaccine for coliform mastitis?

A

J5 strain. Works on other bacteria.