Collection and Handling of Biological Samples Flashcards Preview

RUSVM Clinical Pathology > Collection and Handling of Biological Samples > Flashcards

Flashcards in Collection and Handling of Biological Samples Deck (9)
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1
Q

Blood for biochemical profile should be analyzed in what time frame?

A

Blood should be allowed to clot for 15-30 minutes

Serum should be separated by centrifugation

Refrigerate serum or freeze if not being analyzed within 2 days

2
Q

Green Top

A

Heparin

Anticoagulant - inhibits thrombin

Contains fibrinogen

Uses: Chemistry, Avian/reptile CBC and chem, Plasma colloid oncotic pressure, measurement of electrolytes, specific tests

3
Q

Tiger Top (Red and Black)

A

Gel that promotes blood clot formation and separates cells from serum

Used for chemistry and serology

4
Q

Blue Top

A

Citrate

Anti-Coagulant, Ca Chelator

Use: Coag tests, PIVKA, Antithrombin, Coag Factor Analysis, Von Willebrands Factor Analysis

5
Q

Gray Top

A

Sodium Fluoride Oxalate

Anti-coagulant, Ca chelator

Inhibits glucose metabolism

Use: serial glucose, lactate and pyruvate

6
Q

Red Top Tube

A

No Anti-Coagulant

Blood is expected to clot

Serum used for biochemical profile and other tests

7
Q

Lavender Top

A

EDTA to K Salt

Anticoagulant and Ca2+ chelator

Preserves cell morphology

Uses: CBC, Fibrinogen, Reticulocyte count, Buffy coat analysis, fluid analysis, blood banks, coombs test, PCR, endogenous ACTH

8
Q

Blood for a CBC should be analyzed in what time frame

A

Within the hour of taking the sample

9
Q

Volume of blood needed for CBC and Biochemical Profile?

A

5 mL