Flashcards in Clinical Pathology Deck (71)
Problems with a slow bleed
Low BP can lead to a slow bleed
Problems with a fast bleed/ high suction
Haemoloysis (cell distortion)
Possible vein collapse
Serum is derived from?
How is a sample mixed?
Fine needle aspirate
Are all slides stained inhouse ?
Cell layer thickness cytology slide:
How many 1 sec dips in diff quik
Label parts of sample after 30 mins
- plain tube
- EDTA tube
Plain tube: serum & clotted RBC
EDTA: plasma, buffy coat (WBC, platelets), red cells
What is found in plasma but is not found in serum?
Microscopic fibre formed when blood clots:
Why do we separate blood from clot for long term storage ?
To prevent chemicals leaching out of old and damaged cells
Prac methods of separating serum from clot:
Using pipette to siphon off serum
Serum separator tube (SST)
Most versatile anticoagulant
Best anticoagulant for blood smears in mammals
Use of citrate (blue) and flox (grey) tubes:
Citrate (blue): coag studies
Flox (grey): delayed glucose testing
What happens if serum is centrifuged before clot retraction occurs?
Serum will become a solid fibrinous gel because platelets are spun out before they have contracted fibrin in clot
Where should a sample be left for clotting ?
Room temp for 30mins
Inverted 5 times
Left at room temp to clot
Spun down after 30 mins
Serum poured off within 60 mins
Which blood cells are responsible for clot retraction/ contraction?
Why is it best to fast an animal before collecting blood?
Parts of blood smear
Buffy coat (WBC, platelets)
Anaemic dog and cat PCV
Healthy dog and cat PCV
Healthy dog TPP
Read meniscus at ?