Flashcards in Chapter 10 Genetic Engineering Deck (56)
What best describes a transgenic animal?
An animal whose chromosomes contain DNA from an external source
About 80% of the genes present in humans are also present in the mouse genome.
What term represents the hereditary information that it present in an animal?
What is a transgene?
The fragment of DNA that is introduced into the nucleus of a cell.
Barrier access to an animal room increases the risk of introducing disease entities to valuable genetically modified lines.
Whenever a dam experiences dystocia, oxytocin is effective at inducing labor.
A gene's phenotype develops in utero and can always be observed after birth..
False: it can develop in utero and also during develop or adulthood
Overbreeding to obtain the number of mice needed with a particular genotype is unacceptable in a transgenic animal facility
If a large number of animals must be euthanized because of gene expression is inadequate, animal care staff may need emotional support.
Genetically modified mice have the same housing and nutrition needs as any other mice in the facility.
Every transgenic animal facility should have a mechanism for observing, documenting, and reporting health concerns regarding breeding or unique phenotypes.
With barrier access to a room, investigators must rely on animal care staff to handle all animal needs.
What might affect transgenic mice and the success of their breeding?
Noise, odors, and lighting
Females with a litter from implanted fertilized eggs are called:
What is an initial observation indicating successful mating?
In the production of gene-targeted animals, mating with a stud makes the recipient female pseudopregnant.
Placing pups with a female other than the one who gave birth to them is called cross-fostering.
If fertilized eggs are required, along with egg donor females, what animals are required?
Embryos can be transferred to recipient mice surgically if the embryos are at least 3.5 days old.
False: Embryos can be transferred to recipient mice surgically only if the embryos are less than 3.5 days old.
Pronuclear injection provides the scientist with a more specific tool than gene-targeted mutation for placement of the transgene.
To construct a transgene, the scientist must start with an existing piece of DNA that originated in a living organism.
A transposon is a DNA sequence obtained from bacteria.
Pronuclear injection introduces a transposon-flanked DNA sequence with an enzyme that inserts multiple copies of the DNA into the genome.
False: a single copy
PCR can be used to genotype an animal born after pronuclear injection of a transgene.
If a transgene is intergrated in one of the chromosomes of a pair, the animal is said to be:
What best describes "alleles"?
Copies of the same gene
Wild-type animals are more commonly used to start a transgenic line because it is easier to observe a phenotype change than in inbred strains.
False: inbred strains are used because a phenotypic change is easier to see.
All animals produced by pronuclear injections of the same transgene have the same phenotype.
Offspring of two hemizygous parents inherit at least a copy of the transgene.
False: in theory, 25% of the offspring will have NO copy of the transgene.