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Flashcards in C. elegans methods Deck (14)
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1
Q

First step of washing c. elegans

A

use 1000 microliter M9 buffer 3x

run down plate to scoop c. elegans

2
Q

Do we wash worms with pure water?

A

no

3
Q

Second step of washing c. elegans

A

centrifuge test tube

make sure to balance the centrifugeq

4
Q

How long should you centrifuge?

A

Really quick

a couple of seconds

5
Q

Why not over-centrifuge?

A

Do not want to squish the worms

6
Q

supernatant

A

liquid lying about the pellet after centrifuging

7
Q

Third step of washing c. elegans

A

remove supernatant

add 1ml of M9 onto worms

flick the tube to resuspend the worms

8
Q

Why do we wash?

A

Do not want to replate bacteria

want to wash bacteria and worm waste away

9
Q

Fourth step of washing c. elegans

A

centrifuge again after flicking

and remove the supernatant

repeat

10
Q

fifth step of washing c. elegans

A

resuspend in desired concentration

11
Q

If worms are too concentrated?

A

Add more solution

12
Q

If worms are not concentrated enough

A

re-centrifuge and remove supernatant

13
Q

What concentration did we want?

A

Roughly 40 worms in 5 microliters

14
Q

Benefits of using c. elegans

A

Hermatphoites

Eat bacteria (cheap)

40% same genes as humans

3 week lifesspan

scientists can manipulate the genome