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Flashcards in Biochemistry Deck (372)
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31

In which direction does DNA pol III proofread (Exonuclease Activity)?

3' to 5'

32

What are the two functions of prokaryotic DNA Pol I?

remove RNA primers and replaces it with DNA

33

What is a transition mutation?

purine to purine or pyr. to pyr.

34

What is a transversion mutation?

purine to pyr. or pyr. to purine

35

What DNA replication pathway is defective during xeroderma pigmentosum?

nucleotide excision repair

36

What end does DNA endonuclease cleave?

5'

37

What end does DNA lyase cleave?

3'

38

What DNA repair mechanism is faulty in HNPCC?

mismatch repair

39

What DNA repair mechanism is faulty in ataxia telangiectasia?

non-homologous end joining

40

What position of DNA is the hydroxyl group located?

3' end

41

What binds at the promoter?

RNA polymerase

42

What binds the enhancer?

Tx Factors

43

What binds at the silencer?

negative repressors

44

What enzyme does α-aminitin inhibit?

RNA pol II

45

What does eukaryotic RNA pol I synthesize?

rRNA

46

What does eukaryotic RNA pol II synthesize?

mRNA

47

What does eukaryotic RNA pol III synthesize?

tRNA

48

What three processes are done to mRNA before it leaves the nucleus?

capped, tailed and spliced

49

Is the 5' or 3' end of mRNA capped? What molecule is used as a cap?

5'

7-methylguanosine

50

Is the 5' or 3' end of mRNA polyadenylated?

3' = polyA

51

Where in the cell does translation occur?

cytosol

52

What is the six nucleotide polyadenylation signal?

AAUAAA

53

What are snRNPs involved with?

formation of spliceosome

54

anti-Smith antibodies target what? What disease?

snRNP's

SLE

55

Anti-U1 RNP's are highly indicative of what disease?

mixed connective tissue disease

56

Is the CCA of tRNA on the 3' or 5' end?

3'

57

What is the function of the T-Arm of tRNA? What three bases are carried on the T-arm of tRNA?

to bind tRNA ribosome

Thymine/pseudouridine/Cytosine

58

The D-arm of tRNA contains what base? What is the function of this base?

dihydrouracil

binding of correct aminoacyl-tRNA synthetase

59

Is the CCA of tRNA on the 3' or 5' end?

3'

60

What does aminoacyl tRNA synthetase use for energy?

ATP