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Flashcards in Biochem 2 Deck (10)
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1
Q

Restriction enzymes

A

PALINDROMIC

5’ to 3’ same in both strands

2
Q

cDNA

A
  • only coding regions (Vs genomic library) from mRNA

- recombinant proteins

3
Q

recombinant proteins are from

A

cDNA

4
Q

lactose

A

galactose-B-1,4-glucose

5
Q

What type of inhibition can be overcome by excess substrate?

A

Competitive

Noncompetitive binds equally to E and E-S
Uncomp solely E-S

6
Q

Antiaromatic

A
  • unstable/reactive
  • cyclic, PLANAR, conjugated
  • 4n (add 2 for aromatic)
7
Q

Pressure / moles of gas equilbrium

A

-increasing pressure shifts rxn towards fewer moles of gas

8
Q

Doing what to blood vessels increases oxyhemoglobin?

A

vasoconstriction

Hb + O2 -> HbO2

Shifting to fewer moles of gas = increase pressure = constrict

9
Q

intrinsic factors bind B12 better in what kind of environment?

A

alkaline

10
Q

postprandial state

A

well-fed state

nutrients flow through liver first via hepatic portal vein
lasts 3-5 hours after meal
marked by anabolism + fuel storage