BB Ch21

Question 1

2) The most difficult parameter to control is:
a. Temperature
b. Cage size
c. Humidity

Answer 1

c

Question 2

3) An environmental factor that may influence experimental data is:
a. Physical
b. Chemical
c. Microbial
d. All of the above

Answer 2

d, duh

Question 3

4) T or F A dual-corridor circulation pattern in the animal caging area is better than a single-corridor pattern to reduce chances of cross contamination.

Answer 3

True

Question 4

5) The most common cubical size is:
a. 4 feet deep by 6 feet wide
b. 7 feet deep by 7 feet wide
c. 7 feet deep by 10 feet wide

Answer 4

a

Question 5

6) What percentage of the mobile cage racks should the dirty side of the cage sanitizer hold?

Answer 5

at least 10%

Question 6

7) The maximum temperature that feed from natural sources should be stored at is:

Answer 6

70 F (21 C)

Question 7

8) T or F An ABSL-2 room holding nonhuman primates should maintain negative pressure relative to the corridor.

Answer 7

True, duh

Question 8

9) What does the acronym HEPA stand for?

Answer 8

High Efficiency Particulate Air

Question 9

10) The official definition of a HEPA filter is a filter that removes:

Answer 9

99.97% of particles measuring 0.3 um in diameter

Question 10

11) T or F Air supplied to animal rooms should be 100% outside air.

Answer 10

True

Question 11

12) In a single-corridor conventional facility holding clean and dirty animals, the rooms with the “clean” animals should have a (positive or negative) air balance?

Answer 11

positive

Question 12

13) The standard design temperature range for animal rooms is:

Answer 12

65-85 F

Question 13

14) The generally accepted range for relative humidity control is:

Answer 13

30-70%, duh

Question 14

15) T or F The “Guide” recommends that rooms housing albino animals be approximately 325 lux (30 foot-candles) at 1.0 m (3.3 ft) above the floor. This level of light appears to be adequate for routine animal care.

Answer 14

True (verify)

Question 15

16) Floor drains in animal rooms should be a minimum of what size?

Answer 15

4” for rodent rooms and 6” for rooms that flush waste (verify)

Question 16

What is the minimum recommended ceiling height?

Answer 16

9 ft

May need to be taller if rodent caging is expected to exceed 7-8 ft.

Question 17

18) The recommended door size for rooms housing nonhuman primates is:

Answer 17

42” wide x 8’ tall

Question 18

T or F Fire alarms that operate between 400 kHz and 500 kHz should be used in facilities housing rodents because most rodent species cannot hear frequencies below 1000 kHz.

Answer 18

True

Question 19

According to the “Guide”, corridors should be:

Answer 19

6-8 ft wide

Question 20

21) Sanitation can be achieved at:

Answer 20

180° F for 1 second or
161° F for 15 seconds or
143° F for 30 minutes

Question 21

22) T or F Hyperchlorination has been widely used in the past, but the most common water treatment used for controlling microbial contaminants delivered via water bottles is acidification to a pH of 2.5 -3.0.

Answer 21

True

Question 22

23) T or F The standard practice is to change cages 1 time per week when using a ventilated microisolation caging system.

Answer 22

True

Question 23

Which type of Biological Safety Cabinet is most commonly used in research animal facilities?

Answer 23

Class II

Question 24

25) T or F It is required by the Animal Welfare Act that training must be provided for personnel caring for laboratory animals.

Answer 24

True, duh

Question 25

26) The LAM book recommends that SOP’s be reviewed how often?

Answer 25

b. Preferably every 6 months, but no less that once a year

Question 26

27) Name 2 types of cage washers used.

Answer 26

27) Tunnel, Batch washers

Question 27

28) Name 3 types of animal diets.

Answer 27

28) Closed formula, Open formula, Certified

Question 28

29) What are the two main types of bedding used?

Answer 28

29) Contact, Non-contact

Question 29

30) In general, how often should animal waste be washed down and primary enclosures sanitized?

Answer 29

Wash down runs daily, Sanitize every 2 weeks