Antibody Flashcards Preview

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Flashcards in Antibody Deck (40)
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1
Q

H chain - define

A

heavy chain - 1 variable region 3-4 constant region

2
Q

L chain - define

A

light chain - 1 variable region 1 constant region

3
Q

Kappa chains - define

A

Type of L chain

4
Q

Lamda chains - define

A

type of L chain

5
Q

Fab - define

A

Fragment antibody - contains the variable L/H + CL/H (only one control domain

6
Q

Fc - define

A

Fragment constant - only the heavy chains’ CH2, 3, (4)

7
Q

Put in order on electrophretic separation:

Anode, cathode, albumin, alpha 1, alpha 2, beta, gamma peaks

A

Cathode | albumin, alpha 1, alpha 2, beta 1, beta 2, gamma | Anode

8
Q

Heavy chain for IgG, IgE, IgD, IgA, IgM

A

G - 2 gamma chains
E - 2 epsiolon CH4 (4th domain) - affinity for mast cells (histamine) - allergies
D - 2 delta chains for baby cells - receptor molecule - note extra long hinge
A: 4 alpha chains, 1 J, and 1 S.C. chain
M: 10 mu, 1 J ring/chain

9
Q

Hinge

A

Between Fab/Fc - can change conformation

10
Q

Ig_ has secretory chain

A

IgA

11
Q

Order immunoglobins classes from largest to smallest

A

M, A, E, D, G

12
Q

Antibody combining site

A

3 CDR on each light/heavy chains

Once bound –> allosteric changes. Fc changes structurally

13
Q

CDR

A

complementary determining region - also hypervariable region - random splicing

14
Q

Allotype

A

minor allectic difference in sequence of immunoglobulins (ie. Ala vs Tyr on specific spot on heavy chain)

15
Q

Idiotype

A

antibody with unique CDR - if sequence is recognized by another antibody- will behave as an antigen (Ab behaves as an Ag)

16
Q

IgG is ___ valent?

A

Di

17
Q

Immune complex can happen when:

A

antibody cross linking two antigen

18
Q

Aggregation –> fall out of solution. Antigen is a molecule, aggregation is called ______; antigen is a cell, aggregation is called a ____

A

precipitation, agglutination

19
Q

Ig_ is made by fetus

A

M

20
Q

Ig_ cross placenta via _________________

A

IgG –> placenta via active transport

21
Q

Ig_ is decavalent, can activate complement with # antibody

A

M

1

22
Q

Ig_ needs __ antibody to complement

A

G, 2

23
Q

IgG has half life of…

A

3 weeks

24
Q

Phagocytic cells have FcR for Ig_ –> opsonizing

A

IgG

25
Q

Ig_ is most common in blood

A

G

26
Q

Ig_ in mucosa

A

A

27
Q

Ig_ is first line of immunological defense

A

A

28
Q

Ig_ for B cell receptors

A

D

29
Q

Ig_ is responsible allergies/hypersensitivity

A

E

30
Q

Ig_ is for ____ cells

A

mast/basophils (have FcR for IgE) –> histamines

31
Q

precipitation of antigen/antibody will only form if they are _______ because of ______

A

Equal in concentration

Precipitation/agglutination

32
Q

Complement for innate and adaptive immunity

A

Innate - lectin

Adaptive - classical

33
Q

Order of complement via classical

A

C1q –> 4, 2, 3, 5,6,7,8,9

34
Q

Order of alternative complement

A

Cell wall = P
P –> stablize C3b and anchor for factor B and D –> C3BDbC3b –> C5 –> 6,7,8,9
Autoimmunity

35
Q

Lectin pathway - order

A

MBP is a lectin - binds foreign carbs

MBP –> Carbs –> 4,2,3,5.6,7,8,9

36
Q

C_ and C_ does lytic

A

8,9

37
Q

which complement components opsoninze?

A

C3b;

IgG opsonize for FcR of phagocytes

38
Q

Chemotatic complement component

A

C5a

39
Q

Anaphylatoxic complement component

A

C3a, C4a, C5a –> release histamine from mast/basophil –> increase inflammation –> increase blood flow

40
Q

Lysis important for what family of bacterial

A

Neisseria (gonorrhrea + meningitis)

Reisstant for lysis –> phaogcytosed