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Flashcards in Animal lab procedures lab Deck (299)
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1

The magnification of the high dry objective is

40x

2

The condenser and diaphragm are used to

take full advantage of the microscope's resolving capacity and adjust the amount of light

3

T/F more light is needed when evaluating a direct fecal smear than when using oil immersion

false

4

Purple tops contain

EDTA

5

What is hemolysis?

ruptured RBCs

6

How can one tell is hemolysis has occurred?

any variation of pink to red

7

T/F heparin imparts the best preservation of cellular morphologic detail

false

8

Serum/plasma that is opaque and "milky" is called _____

lipemia

9

What causes serum/plasma to be lipemic?

fat in the blood

10

Why should blood films be prepared immediately after blood collection?

to reduce artifacts in cell morphology caused by anticoagulants

11

What is the most common cause of a falsely elevated PCV?

not spun for 5 minutes

12

When we measure the PCV column on a spun microhematocrit what are we assessing in the animal?

RBC, or possibly anemia

13

If a dog has a PCV of 33% what is its est hemoglobin value?

11 g/dl

14

List 4 different parameters that can be evaluated by spinning whole blood in a microhematocrit tube

est hemoglobin, tp, pcv, wbc

15

List the types of cells found in whole blood

RBCs, WBCS

16

What is rouleaux?

aggregation of RBC into linear formations resembling stacks of poker chips

17

What is agglutination?

aggregation of RBC into grapelike clusters

18

What is left shift?

increased number of immature neutrophils

19

What is right shift and what causes it?

increased number of hypermature neutrophils, aging

20

What are reactive lymphocytes?

lymphocytes with certain morpholic changes

21

What is monocytosis and what causes it?

(immature macrophil) increased number of monocytes, stress or necrosis

22

What is lymphocytosis?

(epinephrin)increased number of lymphocytes

23

What is lymphopenia?

decreased number of lymphocytes

24

What is eosinphilia and what causes it?

increased number of eosinophils caused by allergic reactions

25

What are some variables that affect the results of a fecal examination?

history of the patient (recent travel), timing (on heartworm preventative?), proper sample size ( 1g of fecal required), specific gravity for the desired flotation media (check solution routinely)

26

What is the specific gravity of the floatation media used in lab (fecasol)?

1.25-1.30

27

______ has a higher specific gravity than the eggs in a fecal

solution

28

How do you make a direct smear?

drop of saline on a slide, mix feces to saline, place coverslip over the drop

29

How do you make a centrifugal flotation?

prepare fecal using 2-5g, strain, fill tube, place in centrifuge for 10 mins, take out wait 5 mins, coverslip on slide

30

What do you do to encourage clients to control measures of parasites?

remove feces from the yard and play areas, wash hands following pet contact, clean dogs and cats promptly, feed only cooked or prepared food