The magnification of the high dry objective is
The condenser and diaphragm are used to
take full advantage of the microscope's resolving capacity and adjust the amount of light
T/F more light is needed when evaluating a direct fecal smear than when using oil immersion
Purple tops contain
What is hemolysis?
How can one tell is hemolysis has occurred?
any variation of pink to red
T/F heparin imparts the best preservation of cellular morphologic detail
Serum/plasma that is opaque and "milky" is called _____
What causes serum/plasma to be lipemic?
fat in the blood
Why should blood films be prepared immediately after blood collection?
to reduce artifacts in cell morphology caused by anticoagulants
What is the most common cause of a falsely elevated PCV?
not spun for 5 minutes
When we measure the PCV column on a spun microhematocrit what are we assessing in the animal?
RBC, or possibly anemia
If a dog has a PCV of 33% what is its est hemoglobin value?
List 4 different parameters that can be evaluated by spinning whole blood in a microhematocrit tube
est hemoglobin, tp, pcv, wbc
List the types of cells found in whole blood
What is rouleaux?
aggregation of RBC into linear formations resembling stacks of poker chips
What is agglutination?
aggregation of RBC into grapelike clusters
What is left shift?
increased number of immature neutrophils
What is right shift and what causes it?
increased number of hypermature neutrophils, aging
What are reactive lymphocytes?
lymphocytes with certain morpholic changes
What is monocytosis and what causes it?
(immature macrophil) increased number of monocytes, stress or necrosis
What is lymphocytosis?
(epinephrin)increased number of lymphocytes
What is lymphopenia?
decreased number of lymphocytes
What is eosinphilia and what causes it?
increased number of eosinophils caused by allergic reactions
What are some variables that affect the results of a fecal examination?
history of the patient (recent travel), timing (on heartworm preventative?), proper sample size ( 1g of fecal required), specific gravity for the desired flotation media (check solution routinely)
What is the specific gravity of the floatation media used in lab (fecasol)?
______ has a higher specific gravity than the eggs in a fecal
How do you make a direct smear?
drop of saline on a slide, mix feces to saline, place coverslip over the drop
How do you make a centrifugal flotation?
prepare fecal using 2-5g, strain, fill tube, place in centrifuge for 10 mins, take out wait 5 mins, coverslip on slide
What do you do to encourage clients to control measures of parasites?
remove feces from the yard and play areas, wash hands following pet contact, clean dogs and cats promptly, feed only cooked or prepared food