2.4 Catalase Enzyme Experiment Flashcards Preview

Biology Chapter 1 (AQA) > 2.4 Catalase Enzyme Experiment > Flashcards

Flashcards in 2.4 Catalase Enzyme Experiment Deck (15)
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1
Q

What is the IV in this experiment?

A

Temperature

2
Q

What is the DV in this experiment?

A

Volume of O2

3
Q

What are other variables that need to be controlled?

A

PH
Substrate concentration
Enzyme concentration

4
Q
  1. Set up boiling tubes with same volume and concentration of what?
A

Hydrogen peroxide

5
Q
  1. How do you keep PH constant, what does this do?
A

Adding buffer solution

Resists changes in PH when small amounts of acids and alkalis are added

6
Q
  1. What apparatus is set up to measure volume of O2 production?
A

Delivery tube and upside down measuring cylinder

7
Q
  1. Each boiling tube containing hydrogen peroxide is put where?
A

Water bath

8
Q
  1. What is different about each water bath?
A

Each set to different temperature

9
Q
  1. What else is put in a water bath other than hydrogen peroxide solution?
A

Tube containing catalase

10
Q
  1. How long do you wait for each enzyme to reach set temperature before removing from water bath?
A

5 minutes

11
Q
  1. Then use a pipette to add what?
A

The same volume and concentration of catalase to each tube

12
Q
  1. The record O2 production in the …
A

First minute reaction

13
Q
  1. How many times do you repeat the experiment?
A

3 times at each temperature

14
Q
  1. What do you then use the results to calculate?
A

Mean O2 production at each temperature

15
Q
  1. You can then calculate mean rate of reaction at each temperature, what is the calculation for this?
A

Volume of O2 produced ÷ time taken (cm3s-1)