2014 biochem midterm Flashcards

1
Q

what consequence is expected from an enzyme that shows multiple interacting substrate binding site?

a. hyperbola curve
b. Sigmoid co-operative
c. allosteric site active

A

b. Sigmoid co-operative

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2
Q

At what stage is DNA synthesized in a PCR reaction?
a- annealing
b- denaturation
c- extension

A

c- extension

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3
Q
what bonds are disrupted when you boil an egg?
a- amide linkage 
b- peptide
c- non-covalent bond
d- s-s bonds
A

c- non-covalent bond

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4
Q
what causes a frameshift mutation?
a-deletion
b-translocation
c-inversion
d-duplication
A

a-deletion

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5
Q

What differentiates a prokaryotic gene from a eukaryotic in terms of gene structure?

A

Absence of introns

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6
Q

Which is a characteristic of chromatin remodeling
a- occurs in the nucleus
b- regulate gene transcription
c- important for DNA polymerase

A

b- regulate gene transcription

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7
Q

what is the percentage of non-relative DNA?

a. 40%
b. 45%
c. 50%
d. 55%

A

b. 45%

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8
Q

what is a characteristic of B-turn?

A

caused by proline residue

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9
Q

what is the primary sensor that recognizes incorrect protein folding?

a. glucosyltransferase
b. glucosidase I
c. glucosidase II

A

a. glucosyltransferase

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10
Q

what genetic change is universal in gene silencing?

a. Phosphorylation
b. DNA methylation

A

b. DNA methylation

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11
Q
in which nucleotide sequencing of the proximal DNA sequencing does methylation most commonly occur?
a-AMP
b-GMP
c-CMP
d-TMP
A

c-CMP

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12
Q

what is the major difference between normal proline and toxic proline?
a-Primary
b-Secondary

A

b-Secondary

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13
Q

what stimulates glycogen synthesis in muscle?
a-insulin
b-glucagon

A

a-insulin

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14
Q

What’s responsible for elongation?
a-A-site
b-P-site
c-E-site

A

a-A-site

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15
Q

What is the function of copper in collagen structure?

A

Cross-linking

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16
Q

Reversible competitive inhibitor effect?
a-increase Km
b-increase Vmax
c-increase delta G

A

a-increase Km

17
Q

which of the following is a characteristic of primary protein?

a. Disulfur bond
b. Linear AA residues
c. Hydrogen bond

A

b. Linear AA residues

18
Q

What is the characteristic of m-RNA editing?

A

Change in the m-RNA sequence

19
Q

What is the role of ddNTP in DNA sequencing?

A

Base selective inhibition