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FB1 - 2nd year > 2012 biochem midterm > Flashcards

2012 biochem midterm Flashcards

1
Q

Which Histone is NOT present in the nucleosome core?

a. H1
b. H2
c. H3
d. H4
e. H5

A

a. H1

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2
Q

We have 1 primer amplified within 20 cycles of PCR techniques. How many folds do we have?

a. 20 folds
b. 200 folds
c. 2,000 folds
d. 20,000 folds
e. 200,000 folds

A

a. 20 folds

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3
Q

What process will be affected if a mutation occurs in the census sequence AAUAAA?

a. 5’ Capping
b. Addition of 3’ Poly-A-tail
c. Splicing
d. Removal of Introns
e. RNA Initiation

A

b. Addition of 3’ Poly-A-tail

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4
Q

Taking gene from one person & incorporating it with another person. What is it called?

a. Recombinant DNA
b. Restriction modification
c. DNA mutation
d. DNA signaling

A

a. Recombinant DNA

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5
Q 
What’s the property of Collagen?
a. Hypo-methylated 
b. Hyper-methylated
C. Contain 2 identical polypeptide chain
D. Heavily glycosylated
E. Stabilized by hydrophobic interactions
A

D. Heavily glycosylated

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6
Q

Why’s the reaction stopped when we used ddNTP?

a. 3’-OH is used
b. 3’-H is used
c. Primer is hydrolyzed
d. The annealing temperature is too high

A

b. 3’-H is used

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7
Q

What disturbs the secondary structure of the protein?

a. Lysine
b. Isolysine
c. Proline
d. Glycine

A

c. Proline

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8
Q

Gum Bleeding is caused by a deficiency of Vitamin C. What biochemical change will occur?

a. A decrease in the hydroxylation of Proline
b. A decrease in the hydroxylation of Lysine
c. A decrease in the hydroxylation of Glycine

A

a. A decrease in the hydroxylation of Proline

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9
Q

Lack of which OH prevent ddNTP?

a. 1’-OH
b. 2’-OH
c. 3’-OH
d. 4’-OH
e. 5’-OH

A

c. 3’-OH

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10
Q

What helps insert a protein with M6P in Golgi?

a. Lysosomes
b. Cytoplasm
c. Membrane
d. ER
e. Receptor

A

a. Lysosomes

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11
Q

Aminoacyl tRNA (aa-tRNA) that carry amino acid to translation are highly reactive. What factor stabilizes them in the cytoplasm?

a. EFG
b. EF-1
c. EF-3
d. Tu
e. Ts

A

d. Tu

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12
Q

How do suicide enzymes affect substrate?

a. Bind to allosteric site
b. Bind to active site
c. Disrupt synthesis
d. Disrupt degradation

A

b. Bind to active site

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13
Q

What stimulates glycogen synthesis in muscles?

a. Ca
b. Epinephrine
c. Norepinephrine
d. Insulin
e. Glucogon

A

d. Insulin

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14
Q

What’s the key feature in prion structures?

a. Change in primary protein
b. Excessive beta sheets
c. Increase in alpha-helix
d. Decrease in proline

A

b. Excessive beta sheets

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15
Q

What’s the property of highly substrate active sites?

a. High Km
b. Vm
c. Sigmoid-shaped

A

c. Sigmoid-shaped

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16
Q

Who has the highest % of having CF?

a. White
b. Hispanic
c. Black
d. Asian

A

a. White

17
Q

During protein translation, an antibiotic analog aa-tRNA occupies the P-site. What’s inhibited?

a. Elongation
b. Initiation
c. Termination
d. Translocation

A

b. Initiation

18
Q

A gene coding for lysosomal proteins is missing leader sequence. Where will the protein go?

a. Cytoplasm
b. Secretory
c. Mitochondria
d. Nucleus
e. Lysosome

A

e. Lysosome

19
Q

What’s the indication of the change of β-Hemoglobin to sickle cell anemia?

A

Mutation of one AA

20
Q

What kind of mutation does the gene encoding phenylalanine hydroxylase enzyme have?

a. Deletion
b. Splice
c. Nonsense
d. Insertion
e. Missense

A

e. Missense

21
Q

What’s the characteristic of CREB?

A

Transcription Factor

22
Q

Which enzyme activity signals Energy cell deficiency?

a. Na/K ATPase
b. hexokinase
c. AMPase
d. Glycogen synthesis
e. Protein Kinase A

A

a. Na/K ATPase

23
Q

What’s the type of inheritance of CF?

a. X-linked Recessive
b. Autosomal Dominant
c. Autosomal Recessive
d. X-linked Dominant

A

c. Autosomal Recessive

24
Q

What is the characteristic of metaphasic chromosome?

a. Naked DNA molecule w/o any histones
b. Single Stranded DNA Molecule
c. A DNA molecule that is synthesized at the G1 stage of cell cycle
d. 4 strands of DNA with histones
e. 1 strand of DNA with histones

A

d. 4 strands of DNA with histones

25
Q

What’s the characteristic of HK (housekeeping gene)?

a. Continuously expressed
b. Regulated
c. Highly methylated Poorly acetylated
d. Highly phosphorylated

A

a. Continuously expressed

26
Q

In CF, What’s the % of the affected offspring if the 2 parents were carriers?

a. 0
b. 25
c. 50
d. 75
e. 100

A

b. 25

27
Q

Which DNA sequence is used for DNA fingerprinting?

a. Microsatellite DNA
b. Promoter region
c. Hypervariable minisatellite
d. Telomeric DNA Coding DNA

A

c. Hypervariable minisatellite

28
Q

Which of the following is a characteristic of actively transcribed gene?

a. Found in euchromatic
b. Insensitive to α-amantin
c. Transcribed DNA Polymerase
d. Poorly acetylated

A

a. Found in euchromatic

29
Q

What general characteristic is shared by most highly regulated intracellular enzymes?

a. Catalyze fastest step in the pathway
b. Rapidly degraded in the cytosol
c. Can be applied to michaelis-menten equation
d. Unchanged by phosphorylation

A

b. Rapidly degraded in the cytosol

30
Q

What’s the function of a restriction enzyme?

a. Prevent formation of sugar-phosphate backbone
b. Breakdown DNA & RNA into nucleotides
c. Prevent formation of chemical bonds between pairs of nucleotides
d. Synthesis of DNA during replication
b. Breakdown of DNA molecules between specific nucleotides

A

b. Breakdown of DNA molecules between specific nucleotides

31
Q

Phenylketonuria is what type of inborn error of metabolism?

a. Amino acid
b. Lipids
c. Carbohydrates
d. Purine
e. Pyrimidine

A

a. Amino acid

32
Q

What is the characteristic of Transcription initiation factor?

a. Cis-acting
b. Trans-acting
c. Regulating mRNA splicing
d. Multibinding sites
e. Forming RNA-protein complexes

A

b. Trans-acting

33
Q

What is necessary for cooperativity in enzymes?

a. Multiple substrate binding sites
b. Chaperone proteins
c. Allosteric binding sites

A

a. Multiple substrate binding sites

34
Q

What’s the main function of DNA Polymerase?

a. Add nucleotides to free 3’OH end of DNA
b. Act as helicase enzyme
c. Involves in DNA Repair

A

a. Add nucleotides to free 3’OH end of DNA

35
Q

What’s a characteristic of Proto-Oncogenes?

a. activated genes
b. cancer-causing genes
c. cellular gene that has the potential to become cancerous

A

c. cellular gene that has the potential to become cancerous

36
Q

What’s the primer of glycogen synthesis?

A

Glycogenin

37
Q

What’s the role of nucleotide analogs in cancer treatment?

a. Inhibit protein synthesis
b. Inhibit DNA Synthesis
c. Inhibit cell growth

A

b. Inhibit DNA Synthesis

FB1 - 2nd year (42 decks)

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